| Structures of oligosaccharides on beta-galactosidase from Aspergillus oryzae. | |
| | |
MedLine Citation:
|
PMID: 3117779 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The carbohydrate portions of beta-galactosidase from Aspergillus oryzae were found to be composed of two types of sugar chains. They were released equally well with endo-beta-N-acetylglucosaminidase H, but were distinct in their chain length. The long sugar chains (fraction I), corresponding to 4% of the total carbohydrate chains, were composed of galactomannan-type oligosaccharides, which consisted of mannose, galactose, glucose, and glucosamine in the molar ratios of 30.0, 16.4, 1.4, and 2.1 per mol of aspartic acid, respectively. The short sugar chains (fraction II), corresponding to 96% of the total carbohydrate chains, consisted of mannose, galactose, glucose, and glucosamine in the molar ratios of 9.4, 0.6, 0.3, and 1.7 per mol of aspartic acid, respectively. Both types of sugar chains were fractionated into neutral and acidic subfractions. The neutral subfraction of fraction I (I-N), corresponding to 1% of the total carbohydrate chains, was very heterogeneous in length and was resistant to digestion with alpha-mannosidase and beta-galactosidase. The neutral subfraction of fraction II (II-N), corresponding to 91% of the total carbohydrate, was composed of a mixture of oligosaccharides with oligomanneoside chains (Mann GlcNAcol). The major components were similar to high mannose-type oligosaccharides of mammalian origin in their composition and size (n = 5-9). However, digestion of II-N with alpha 1,2-mannosidase produced considerable amounts of Man6GlcNAcol, an unusual product in the case of high mannose-type oligosaccharides of mammalian origin, in addition to the common one, Man5GlcNAcol. |
| | |
Authors:
|
Y Nakao; Y Kozutsumi; I Funakoshi; T Kawasaki; I Yamashina; J H Mutsaers; H Van Halbeek; J F Vliegenthart |
Related Documents
:
|
952929 - Branched-chain amino acid oxidation by isolated rat tissue preparations. 15930469 - Branched-chain [corrected] amino acid metabolism: implications for establishing safe in... 12007739 - Mechanisms of lipoapoptosis: implications for human heart disease. 8921849 - Cloning and sequencing of the ilvbnc gene cluster from mycobacterium avium. 2844699 - Radioiodinated p-phenylene bridged fatty acids as new myocardial imaging agents: synthe... 18555679 - Long-chain fatty alcohols from pomace olive oil modulate the release of proinflammatory... 3173129 - The acid phosphatases of thermoascus crustaceus, a thermophilic fungus. 21152399 - Novel bioluminescent quantitative detection of nucleic acid amplification in real-time. 20064499 - A validated assay for the quantitative analysis of tranexamic acid in human serum by li... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of biochemistry Volume: 102 ISSN: 0021-924X ISO Abbreviation: J. Biochem. Publication Date: 1987 Jul |
Date Detail:
|
Created Date: 1987-12-14 Completed Date: 1987-12-14 Revised Date: 2007-12-19 |
Medline Journal Info:
|
Nlm Unique ID: 0376600 Medline TA: J Biochem Country: JAPAN |
Other Details:
|
Languages: eng Pagination: 171-9 Citation Subset: IM |
Affiliation:
|
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acids
/
analysis Aspergillus / enzymology* Aspergillus oryzae / enzymology* Carbohydrate Sequence Galactosidases / isolation & purification* Glycopeptides / isolation & purification Molecular Sequence Data Oligosaccharides / isolation & purification* beta-Galactosidase / isolation & purification* |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acids; 0/Glycopeptides; 0/Oligosaccharides; EC 3.2.1.-/Galactosidases; EC 3.2.1.23/beta-Galactosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Absence of penicillin-binding protein 4 from an apparently normal strain of Bacillus subtilis.
Next Document: A positive regulatory sequence of the Saccharomyces cerevisiae ENO1 gene.