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Structures of nucleolus and transcription sites of rRNA genes in rat liver cells.
MedLine Citation:
PMID:  18726386     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
We observed the ultrastructure of nucleolus in rat liver cells by conventional electron microscopy, and employed cytochemistry NAMA-Ur DNA specific stain method to analyze the distribution and position of nucleolar DNAin situ. The results showed that nucleolar DNA of rat liver cells comes from nucleolus-associated chromatin, and continuously extends in the dense fibrillar component (DFC) of nucleolus, localizes at the periphery of fibrillar center (FC) and in DFC. Furthermore, by employing anti-DNA/RNA hybrid antibodies, we directly and selectively labeled transcription sites of rRNA genes and testified that localization of transcription sites not only to DFC but also to the periphery of FC.
Authors:
W Tao; M Jiao; J He; M He; S Hao
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Science in China. Series C, Life sciences / Chinese Academy of Sciences     Volume:  43     ISSN:  1006-9305     ISO Abbreviation:  Sci. China, C, Life Sci.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2008-08-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9611809     Medline TA:  Sci China C Life Sci     Country:  China    
Other Details:
Languages:  eng     Pagination:  302-9     Citation Subset:  -    
Affiliation:
Institute of Genetics and Cytology, Northeast Normal University, 130024, Changchun, China.
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