Document Detail

Structures of the N-linked carbohydrate of ascorbic acid oxidase from zucchini.
MedLine Citation:
PMID:  9530960     Owner:  NLM     Status:  MEDLINE    
The N-glycan moiety of ascorbic acid oxidase from zucchini (Cucurbita pepo) has been described to be a core-pentasaccharide with a xylose [D'Andrea et al. (1988) Glycoconjugate J 5:151-7]. Ascorbic acid oxidase is sometimes used to characterize antibodies directed against carbohydrate determinants on plant glycoproteins. To prevent misinterpretations of immunological data, the structure of the N-glycan of ascorbic acid oxidase has been reinvestigated. The oligosaccharides were released by almond N-glycosidase and analysed as their pyridylamino derivatives by 2D-HPLC and exoglycosidase digestions. The main structure resembled the typical complex plant N-glycan consisting of a core-pentasaccharide decorated with xylose and 3-linked fucose. The other abundant species lacked the fucose residue. Small amounts of these glycans carried a GlcNAc residue on the 6-arm. Therefore, ascorbic acid oxidase will not only react with antibodies directed against the xylosylated region but also with those binding to N-glycans with 3-linked fucose.
F Altmann
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Glycoconjugate journal     Volume:  15     ISSN:  0282-0080     ISO Abbreviation:  Glycoconj. J.     Publication Date:  1998 Jan 
Date Detail:
Created Date:  1998-05-06     Completed Date:  1998-05-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8603310     Medline TA:  Glycoconj J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  79-82     Citation Subset:  IM    
Institut für Chemie, Universität für Bodenkultur, Wien, Austria.
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MeSH Terms
Ascorbate Oxidase / chemistry*
Carbohydrate Sequence
Cucurbitaceae / enzymology*
Glycoproteins / chemistry*
Molecular Sequence Data
Plant Proteins / chemistry
Polysaccharides / chemistry
Reg. No./Substance:
0/Glycoproteins; 0/Plant Proteins; 0/Polysaccharides; EC Oxidase

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