Document Detail


Structured coculture of mesenchymal stem cells and disc cells enhances differentiation and proliferation.
MedLine Citation:
PMID:  22378296     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: During in vivo stem cell differentiation, mature cells often induce the differentiation of nearby stem cells. Accordingly, prior studies indicate that a randomly mixed coculture can help transform mesenchymal stem cells (MSC) into nucleus pulposus cells (NPC). However, because in vivo signaling typically occurs heterotopically between adjacent cell layers, we hypothesized that a structurally organized coculture between MSC and NPC will result in greater cell differentiation and proliferation over single cell-type controls and cocultures with random organization.
METHODS: We developed a novel bilaminar cell pellet (BCP) system where a sphere of MSC is enclosed in a shell of NPC by successive centrifugation. Controls were made using single cell-type pellets and coculture pellets with random organization. The pellets were evaluated for DNA content, gene expression, and histology.
RESULTS: A bilaminar 3D organization enhanced cell proliferation and differentiation. BCP showed significantly more cell proliferation than pellets with one cell type and those with random organization. Enhanced differentiation of MSC within the BCP pellet relative to single cell-type pellets was demonstrated by quantitative RT-PCR, histology, and in situ hybridization.
CONCLUSIONS: The BCP culture system increases MSC proliferation and differentiation as compared to single cell type or randomly mixed coculture controls.
Authors:
Aliza A Allon; Kristin Butcher; Richard A Schneider; Jeffrey C Lotz
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-03-01
Journal Detail:
Title:  Cells, tissues, organs     Volume:  196     ISSN:  1422-6421     ISO Abbreviation:  Cells Tissues Organs (Print)     Publication Date:  2012  
Date Detail:
Created Date:  2012-07-04     Completed Date:  2013-01-17     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  100883360     Medline TA:  Cells Tissues Organs     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  99-106     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 S. Karger AG, Basel.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Cell Differentiation / physiology
Cell Growth Processes / physiology
Cells, Cultured
Coculture Techniques
Humans
Immunohistochemistry
Intervertebral Disc / cytology*,  metabolism
Mesenchymal Stromal Cells / cytology*,  metabolism
Proteoglycans / metabolism
Grant Support
ID/Acronym/Agency:
DE016402/DE/NIDCR NIH HHS; R01 AR052712/AR/NIAMS NIH HHS; R01 DE016402/DE/NIDCR NIH HHS; R01AR052712/AR/NIAMS NIH HHS; R21 AR052513/AR/NIAMS NIH HHS; R21 AR052513/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Proteoglycans
Comments/Corrections

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