Document Detail


Structure and variability of the UL149 open reading frame from low-passage clinical isolates of human cytomegalovirus.
MedLine Citation:
PMID:  16169096     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human cytomegalovirus (HCMV), a ubiquitous herpesvirus, causes a lifelong subclinical infection in healthy adults but leads to significant morbidity and mortality in neonates and immunocompromised individuals. A region (referred to as UL/b') present in the Toledo strain of HCMV and low passage clinical isolates contains 19 additional genes, which are absent in the highly passage laboratory strain AD169. One of these genes, UL149 open reading frame, was amplified by PCR and sequenced from isolates obtained from infants with congenital HCMV infection, to determine whether genetic variation of this gene could influence the signs of the virus infection. The major finding is that the UL149 is a variable gene in all 26 clinical isolates, and the sequences from clinical isolates were classified into three major groups. It is concluded that the HCMV UL149 sequence is variable at the nucleotide level and it might play an important role in HCMV infection.
Authors:
Yao-hua Ji; Qiang Ruan; Zheng-rong Sun; Yan-ping Ma; Rong He; Ying Qi; Yu-jing Huang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-09-16
Journal Detail:
Title:  Journal of virological methods     Volume:  131     ISSN:  0166-0934     ISO Abbreviation:  J. Virol. Methods     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2005-12-12     Completed Date:  2006-04-13     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8005839     Medline TA:  J Virol Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  72-7     Citation Subset:  IM    
Affiliation:
Virus Laboratory, 2nd Clinical Hospital, China Medical University, Shenyang 110004, PR China.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Cytomegalovirus / genetics*,  pathogenicity
Cytomegalovirus Infections / virology*
Genetic Variation
Humans
Infant
Molecular Sequence Data
Sequence Alignment
Serial Passage
Viral Proteins / genetics*
Virulence / genetics
Chemical
Reg. No./Substance:
0/Viral Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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