| Structure and transcriptional impact of divergent repetitive elements inserted within Phanerochaete chrysosporium strain RP-78 genes. | |
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MedLine Citation:
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PMID: 17033809 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe the structure, organization, and transcriptional impact of repetitive elements within the lignin-degrading basidiomycete, Phanerochaete chrysosporium. Searches of the P. chrysosporium genome revealed five copies of pce1, a ~1,750-nt non-autonomous, class II element. Alleles encoding a putative glucosyltransferase and a cytochrome P450 harbor pce insertions and produce incomplete transcripts. Class I elements included pcret1, an intact 8.14-kb gypsy-like retrotransposon inserted within a member of the multicopper oxidase gene family. Additionally, we describe a complex insertion of nested transposons within another putative cytochrome P450 gene. The disrupted allele lies within a cluster of >14 genes, all of which encode family 64 cytochrome P450s. Components of the insertion include a disjoint copia-like element, pcret3, the pol domain of a second retroelement, pcret2, and a duplication of an extended ORF of unknown function. As in the case of the pce elements, pcret1 and pcret2/3 insertions are confined to single alleles, transcripts of which are truncated. The corresponding wild-type alleles are apparently unaffected. In aggregate, P. chrysosporium harbors a complex array of repetitive elements, at least five of which directly influence expression of genes within families of structurally related sequences. |
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Authors:
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Luis F Larrondo; Paulo Canessa; Rafael Vicuña; Philip Stewart; Amber Vanden Wymelenberg; Dan Cullen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2006-10-11 |
Journal Detail:
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Title: Molecular genetics and genomics : MGG Volume: 277 ISSN: 1617-4615 ISO Abbreviation: Mol. Genet. Genomics Publication Date: 2007 Jan |
Date Detail:
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Created Date: 2006-12-15 Completed Date: 2007-02-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101093320 Medline TA: Mol Genet Genomics Country: Germany |
Other Details:
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Languages: eng Pagination: 43-55 Citation Subset: IM |
Affiliation:
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Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile and Instituto Milenio de Biología Fundamental y Aplicada, Santiago, Chile. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AY532142; DQ400694; DQ444268; DQ444269; DQ677350 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alleles Base Sequence Cytochrome P-450 Enzyme System / biosynthesis, genetics Fungal Proteins / biosynthesis, genetics* Gene Expression Regulation, Enzymologic / genetics Gene Expression Regulation, Fungal / genetics* Genes, Fungal* Glucosyltransferases / biosynthesis, genetics Lignin / metabolism Molecular Sequence Data Mutagenesis, Insertional* Open Reading Frames / genetics Phanerochaete / enzymology, genetics* Retroelements / genetics* Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Fungal Proteins; 0/Retroelements; 9005-53-2/Lignin; 9035-51-2/Cytochrome P-450 Enzyme System; EC 2.4.1.-/Glucosyltransferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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