Document Detail


Structure and tissue distribution of a trinucleotide-repeat-containing gene (cag-3) expressed specifically in the mouse brain.
MedLine Citation:
PMID:  16404148     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Using in silico approaches and RACE we cloned a full length trinucleotide (CAG) repeat-containing cDNA (cag-3). The cDNA is 2478 bp long and the deduced polypeptide consists of 140 amino acids of which 73 are glutamines. The genomic sequence spans approximately 79 kb on mouse chromosome 7 and the gene is composed of four exons. Standard and real-time PCR analyses of several mouse tissues showed that the gene is exclusively expressed in the brain and is not detected in embryonic stages. Within the brain, it is expressed throughout the forebrain region with predominant expression in the hypothalamus and olfactory bulb and very low levels in the mid- and hindbrain.
Authors:
Jin Woo Ji; Hye Lim Yang; Sun Jung Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecules and cells     Volume:  20     ISSN:  1016-8478     ISO Abbreviation:  Mol. Cells     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2006-01-11     Completed Date:  2006-03-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9610936     Medline TA:  Mol Cells     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  348-53     Citation Subset:  IM    
Affiliation:
Department of Biology, Dongguk University, Seoul 100-715, Korea.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Brain / metabolism*
Cloning, Molecular
DNA, Complementary / metabolism
Gene Expression
Mice
Mice, Inbred ICR
Molecular Sequence Data
Polymerase Chain Reaction
RNA, Messenger / genetics,  metabolism
Trinucleotide Repeats / genetics*
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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