Document Detail


Structure of the thyrotropin receptor and thyroid adenylate cyclase system as determined by target analysis.
MedLine Citation:
PMID:  6098307     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bovine thyroid plasma membranes were irradiated with high-energy electrons. Analysis of the target size of the thyrotropin (TSH) receptor revealed a complex pattern composed of a TSH binding component of 71 000 daltons and a large component (several hundred thousand daltons) that masked some of the binding. Both components were also observed when binding was assayed in the presence of 50 mM NaCl. Membranes preincubated with Mg2+ and 10 microM guanosine 5'-(beta,gamma-imidotriphosphate) [Gpp(NH)p], a persistent activator of adenylate cyclase, also showed the presence of these same components. Although the receptor for TSH has been reported to have some similarities to the receptor for cholera toxin, target analysis of [125I]iodocholera toxin binding was consistent with a single small component about the size of a ganglioside. Measurement of the target size of ground-state, i.e., not preactivated, adenylate cyclase was also carried out. The basal (Mn2+) adenylate cyclase yielded a Mr of 85 000, the smallest unit capable of producing cAMP. The Gpp(NH)p-responsive adenylate cyclase has a Mr of 150 000, which may reflect the contribution of the guanine nucleotide regulatory component to the mass of the active enzyme. A similar size was previously measured for the Gpp(NH)p-preactivated, detergent-solubilized thyroid enzyme [Asbury, R.F., Cook, G.H., & Wolff, J. (1978) J. Biol. Chem. 253, 5286-5292]. Radiation inactivation of the NaF-responsive enzyme indicated two or more components to this activity, the smaller of which (140 000 daltons) was similar in size to the ground-state Gpp(NH)p-responsive enzyme and the larger of which was greater than 10(6) daltons.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
T B Nielsen; Y Totsuka; E S Kempner; J B Field
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemistry     Volume:  23     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1984 Dec 
Date Detail:
Created Date:  1985-04-18     Completed Date:  1985-04-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  6009-16     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenylate Cyclase / metabolism*
Animals
Cattle
Cell Membrane / metabolism
Cholera Toxin / metabolism
Guanylyl Imidodiphosphate / pharmacology
Magnesium / pharmacology
Molecular Weight
Receptors, Cell Surface / drug effects,  metabolism*,  radiation effects
Receptors, Thyrotropin
Sodium Chloride / pharmacology
Thyroid Gland / metabolism*,  radiation effects
Thyrotropin / metabolism,  pharmacology
Grant Support
ID/Acronym/Agency:
AM-26088/AM/NIADDK NIH HHS; AM-27285/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Receptors, Cell Surface; 0/Receptors, Thyrotropin; 34273-04-6/Guanylyl Imidodiphosphate; 7439-95-4/Magnesium; 7647-14-5/Sodium Chloride; 9002-71-5/Thyrotropin; 9012-63-9/Cholera Toxin; EC 4.6.1.1/Adenylate Cyclase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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