Document Detail

Structure of sterol aliphatic chains affects yeast cell shape and cell fusion during mating.
MedLine Citation:
PMID:  20150508     Owner:  NLM     Status:  MEDLINE    
Under mating conditions, yeast cells adopt a characteristic pear-shaped morphology, called a "shmoo," as they project a cell extension toward their mating partners. Mating partners make contact at their shmoo tips, dissolve the intervening cell wall, and fuse their plasma membranes. We identified mutations in ERG4, encoding the enzyme that catalyzes the last step of ergosterol biosynthesis, that impair both shmoo formation and cell fusion. Upon pheromone treatment, erg4Delta mutants polarized growth, lipids, and proteins involved in mating but did not form properly shaped shmoos and fused with low efficiency. Supplementation with ergosterol partially suppressed the shmooing defect but not the cell fusion defect. By contrast, removal of the Erg4 substrate ergosta-5,7,22,24(28)-tetraenol, which accumulates in erg4Delta mutant cells and contains an extra double bond in the aliphatic chain of the sterol, restored both shmooing and cell fusion to wild-type levels. Thus, a two-atom change in the aliphatic moiety of ergosterol is sufficient to obstruct cell shape remodeling and cell fusion.
Pablo S Aguilar; Maxwell G Heiman; Tobias C Walther; Alex Engel; Dominik Schwudke; Nathan Gushwa; Teymuras Kurzchalia; Peter Walter
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-02-11
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  107     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-03     Completed Date:  2010-04-16     Revised Date:  2010-09-28    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4170-5     Citation Subset:  IM    
Departments of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA.
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MeSH Terms
Cell Fusion*
Cell Shape*
Cytochrome P-450 Enzyme System / genetics
Ergosterol / biosynthesis
Gene Deletion
Genes, Fungal
Oxidoreductases / genetics
Saccharomyces cerevisiae / cytology*,  genetics,  physiology
Saccharomyces cerevisiae Proteins / genetics
Grant Support
//Howard Hughes Medical Institute
Reg. No./Substance:
0/Saccharomyces cerevisiae Proteins; 57-87-4/Ergosterol; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.-/Oxidoreductases; EC 1.14.14.-/ERG5 protein, S cerevisiae; EC protein, S cerevisiae

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