Document Detail

Structure and stability of crustacean lipovitellin: influence of lipid content and composition.
MedLine Citation:
PMID:  19879374     Owner:  NLM     Status:  MEDLINE    
Lipovitellin (LV) is essential in crustacean eggs for embryo viability and development. Two LV were isolated from eggs of Macrobrachium borellii. corresponding to early (LVe ) and late (LVl) embryo developing stages. They differ in lipid composition but not in lipid/protein ratio or apoprotein composition. Structural information was obtained by fluorescence spectroscopy, far-UV circular dichroism, partial trypsinolysis and electron microscopy applied to LVe and LVl and two partially delipidated forms of LVe generated by phospholipase A2 (LVp) or Triton X-100 (LVt) treatment. All LV forms contained two apoprotein subunits of 94 and 112 kDa, being the 112k Da subunit more accessible to trypsinolysis in all. Only in LVp, different cleavage sites appeared. Secondary structure was similar in LVe and LVl, but LVp and LVt showed a small increase in beta-sheet at expense of alpha-helix. Electron microscopy revealed a spheroidal morphology in all LV and a decreased size in LVp. Delipidated LVs were more resistant to denaturation with guanidinium-HCl. Acrylamide quenching of tryptophan fluorescence was more efficient in delipidated LVs, probably due to apolipoprotein rearrangement, as reinforced by fluorescence anisotropy. It is concluded that LV stability, shape, and apoprotein conformation is not largely affected by the changes in lipid composition that take place during embryogenesis.
C F Garcia; M Cunningham; J L Soulages; H Heras; H A Garda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology     Volume:  155     ISSN:  1879-1107     ISO Abbreviation:  Comp. Biochem. Physiol. B, Biochem. Mol. Biol.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2009-12-16     Completed Date:  2010-03-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9516061     Medline TA:  Comp Biochem Physiol B Biochem Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  126-31     Citation Subset:  IM    
Instituto de Investigaciones Bioqu?micas de La Plata, calle 60 y 120 s/n (1900) La Plata, Argentina.
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MeSH Terms
Circular Dichroism
Egg Proteins / chemistry*,  metabolism
Electrophoresis, Polyacrylamide Gel
Embryo, Nonmammalian / metabolism,  physiology
Guanidine / pharmacology
Lipid Metabolism
Lipids / analysis*,  chemistry*
Microscopy, Electron
Octoxynol / metabolism
Palaemonidae / chemistry*,  embryology
Phospholipases A2 / metabolism
Protein Denaturation / drug effects
Protein Stability
Solvents / chemistry
Spectrophotometry, Ultraviolet
Trypsin / metabolism
Reg. No./Substance:
0/Egg Proteins; 0/Lipids; 0/Solvents; 113-00-8/Guanidine; 9002-93-1/Octoxynol; 9088-43-1/lipovitellin; EC A2; EC

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