| Structure and stability analysis of cytotoxic complex of camel _-lactalbumin and unsaturated Fatty acids produced at high temperature. | |
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MedLine Citation:
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PMID: 21469752 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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α-Lactalbumin α-La), together with oleic acid can be converted to a complex, which kills tumor cells selectively. Cytotoxic α-La -oleic acid and α-La -linoleic acid complexes were generated by adding fatty acid to camel holo α-La at 60°C (referred to as La-OA-60 and La-LA-60 state, respectively). Structural properties of these complexes were studied and compared to the camel α-La. The experimental results show that linoleic acid induces α-La partial unfolding but oleic acid does not change the protein structure significantly. Also the stability of La-OA-60 and La-LA-60 toward thermal denaturation was measured. The order of temperature at the transition midpoint is as follows: La-LA-60 < La-OA-60 < α-La. La-OA-60 complex inhibited tubulin polymerization in vitro. Although the structures of La-OA-60 and La-LA-60 were different, these two complexes had similar cytotoxic effect to DU145 human prostate cancer cells. Samples of La-OA-60 that have been renatured after denaturation lost the specific biological activity toward tumor cells. |
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Authors:
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M S Atri; A A Saboury; A A Moosavi-Movahedi; B Goliaei; Y Sefidbakht; H H Alijanvand; A Sharifzadeh; A Niasari-Naslaji |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of biomolecular structure & dynamics Volume: 28 ISSN: 1538-0254 ISO Abbreviation: J. Biomol. Struct. Dyn. Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-04-07 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8404176 Medline TA: J Biomol Struct Dyn Country: United States |
Other Details:
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Languages: eng Pagination: 919-28 Citation Subset: IM |
Affiliation:
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Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran. saboury@ut.ac.ir. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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