Document Detail


Structure and significance of N-linked sugar unit of human serum amyloid P component.
MedLine Citation:
PMID:  2138034     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human serum amyloid P component (SAP) was digested with pronase P and a glycopeptide fraction was obtained by gel-permeation chromatography. Carbohydrate and amino-acid composition of the glycopeptide suggested that each subunit of SAP possesses an N-linked glycan, but no O-linked ones. The N-linked oligosaccharide of SAP was obtained by hydrazynolysis. The structure of the oligosaccharide, which was deduced by sequential digestion with exoglycosidases and subsequent gel filtration, was identical or very similar to that of human transferrin. Removal of sialic acids from SAP reduced the calcium-dependent binding activity for agarose by 7%, suggesting the terminal sialic acids were partially responsible for the binding.
Authors:
H Hamazaki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1037     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1990 Mar 
Date Detail:
Created Date:  1990-04-16     Completed Date:  1990-04-16     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  435-8     Citation Subset:  IM    
Affiliation:
Department of Biology, Kitasato University School of Medicine, Kanagawa, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Calcium / pharmacology
Carbohydrate Conformation
Carbohydrate Sequence
Carbohydrates / analysis
Chromatography, Gel
Humans
Mannosidases / metabolism
Molecular Sequence Data
N-Acetylneuraminic Acid
Neuraminidase / metabolism
Pronase / metabolism
Sepharose / metabolism
Serum Amyloid P-Component* / metabolism
Sialic Acids
alpha-Mannosidase
beta-Galactosidase / metabolism
beta-Mannosidase
beta-N-Acetylhexosaminidases / metabolism
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Carbohydrates; 0/Serum Amyloid P-Component; 0/Sialic Acids; 131-48-6/N-Acetylneuraminic Acid; 7440-70-2/Calcium; 9012-36-6/Sepharose; EC 3.2.1.-/Mannosidases; EC 3.2.1.18/Neuraminidase; EC 3.2.1.23/beta-Galactosidase; EC 3.2.1.24/alpha-Mannosidase; EC 3.2.1.25/beta-Mannosidase; EC 3.2.1.52/beta-N-Acetylhexosaminidases; EC 3.4.24.-/Pronase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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