Document Detail


Structure of the sialic acid-containing O-specific polysaccharide from Salmonella enterica serovar Toucra O48 lipopolysaccharide.
MedLine Citation:
PMID:  10824100     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lipopolysaccharide was extracted from cells of Salmonella enterica serovar Toucra O48 and, after mild acid hydrolysis (1% AcOH, 1 h, 100 degrees C or 0.1 M NaOH-AcOH, pH 4.5, 5 h, 100 degrees C), the O-specific polysaccharide was isolated and characterized. The core and an oligosaccharide containing a fragment of the repeating unit linked to the core region were also obtained, depending on hydrolysis conditions. On the basis of sugar and methylation analyses and NMR spectroscopy of the hydrolysis products, the biological repeating unit of the O-specific polysaccharide was shown to be the following trisaccharide: -->4)-alpha-Neup5Ac(2-->3)-L-alpha-FucpNAc(1-->3)-D-beta-Glc pNAc(1--> The polysaccharide O-chain was substituted with a single molar equivalent of O-acetyl group, distributed between the Neu5Ac O-9 and O-7 positions, in an approximate ratio of 7 : 3.
Authors:
A Gamian; C Jones; T Lipiński; A Korzeniowska-Kowal; N Ravenscroft
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  267     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-07-20     Completed Date:  2000-07-20     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  3160-7     Citation Subset:  IM    
Affiliation:
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland. gamian@immuno.iitd.pan.wroc.pl
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MeSH Terms
Descriptor/Qualifier:
Carbohydrate Sequence
Gas Chromatography-Mass Spectrometry
Hydrolysis
Lipopolysaccharides / chemistry*
Magnetic Resonance Spectroscopy
Methylation
Molecular Sequence Data
Molecular Structure
N-Acetylneuraminic Acid / chemistry*
O Antigens / chemistry*
Polysaccharides / chemistry*,  isolation & purification
Salmonella enterica / chemistry*
Chemical
Reg. No./Substance:
0/Lipopolysaccharides; 0/O Antigens; 0/Polysaccharides; 131-48-6/N-Acetylneuraminic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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