Document Detail


Structure of rigid-layer of Rhizobium cell wall. I. Purification on the peptidoglycan from the cellulose microfibrils.
MedLine Citation:
PMID:  6179395     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The bag shaped peptidoglycan layer of Rhizobium cell wall was isolated from intact cells after treatment with sodium dodecylsulfate and trypsin, chymotrypsin or pepsin digestion. Results of chemical analysis of acid hydrolyzed peptidoglycan revealed beside two amino sugars: glucosamine and muramic acid, three major amino acids; alanine, glutamic acid and 2,6-diaminopimelic acid and also significant amount of glucose. Evidence were provided that the polyglucose found in peptidoglycan preparations of three strains of Rhizobium trifolii, one of Rhizobium leguminosarum and one of Rhizobium meliloti consist of cellulose microfibrils. The content of cellulose present in Rhizobium peptidoglycans ranged from 60 to 80%. Methods of peptidoglycan purification from the cellulose microfibrils are described.
Authors:
W Drozański; D Drozańska; Z Lorkiewicz
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Acta microbiologica Polonica     Volume:  30     ISSN:  0137-1320     ISO Abbreviation:  Acta Microbiol. Pol.     Publication Date:  1981  
Date Detail:
Created Date:  1982-09-24     Completed Date:  1982-09-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7610362     Medline TA:  Acta Microbiol Pol     Country:  POLAND    
Other Details:
Languages:  eng     Pagination:  355-70     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Cell Fractionation
Cell Wall / ultrastructure*
Cellulose / isolation & purification*
Endopeptidases
Hexosamines / analysis
Peptidoglycan / isolation & purification*
Rhizobium / ultrastructure*
Sodium Dodecyl Sulfate
Species Specificity
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Hexosamines; 0/Peptidoglycan; 151-21-3/Sodium Dodecyl Sulfate; 9004-34-6/Cellulose; EC 3.4.-/Endopeptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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