Document Detail

Structure and metal-binding properties of lipopolysaccharides from heptoseless mutants of Escherichia coli studied by 13C and 31P nuclear magnetic resonance.
MedLine Citation:
PMID:  6358205     Owner:  NLM     Status:  MEDLINE    
The structure and metal-binding properties of lipopolysaccharides (LPS) from heptoseless mutants of Escherichia coli were studied by 13C and 31P NMR techniques. Carbon-13 NMR spectra were used to determine the linkages and configurations of the saccharide backbone and the types and locations of fatty acyl groups in E. coli LPS. Resonance assignments for native LPS were made by chemical shift correlation with model compounds, deacylated LPS, lipid A, deacylated lipid A, and fatty acids released from LPS by mild alkaline hydrolysis. The 3-deoxy-D-manno-octulosonate (KDO) disaccharide was tentatively assigned the structure KDO alpha 2 leads to 5KDO alpha 2 leads to. The presence of amide- and ester-linked 3-hydroxy and 3-acyloxy fatty acids in native LPS was confirmed directly from the 13C spectrum and evidence is presented for a labile acyl ester at C-3' (GlcNII) of the lipid A moiety. A significant finding was that the KDO disaccharide is linked to the C-6' position of the lipid A moiety, rather than C-3', as previously reported. The effects of binding Ca2+, Cd2+, Yb3+, Gd3+, and La3+ on the 31P NMR spectrum of LPS indicated that the glycosidic diphosphate moiety participates in a high affinity metal-binding site.
S M Strain; S W Fesik; I M Armitage
Related Documents :
4343965 - Role of the penicillin-sensitive transpeptidation reaction in attachment of newly synth...
9057835 - Structural studies of the cell-envelope oligosaccharide from the lipopolysaccharide of ...
1693085 - Studies on the lipopolysaccharide of a virulent and an avirulent strain of vibrio vulni...
16458275 - Structural elucidation of the extracellular and cell-wall teichoic acids of staphylococ...
17428115 - Infantile scurvy: an old diagnosis revisited with a modern dietary twist.
14240945 - Evidence for a tricarboxylic acid cycle in mycoplasma hominis.
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  258     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1983 Nov 
Date Detail:
Created Date:  1984-01-07     Completed Date:  1984-01-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  13466-77     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Carbohydrate Conformation
Carbohydrate Sequence
Escherichia coli / genetics*
Heptoses / genetics*
Lipopolysaccharides* / genetics
Magnetic Resonance Spectroscopy
Metals / metabolism*
Metals, Rare Earth
Grant Support
Reg. No./Substance:
0/Heptoses; 0/Lipopolysaccharides; 0/Metals; 0/Metals, Rare Earth; 7440-43-9/Cadmium; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Properties of a thioredoxin purified from rabbit bone marrow which fails to serve as a hydrogen dono...
Next Document:  Mammalian tissue trypsin-like enzymes. Comparative reactivities of human skin tryptase, human lung t...