Document Detail


Structure and metal-binding properties of lipopolysaccharides from heptoseless mutants of Escherichia coli studied by 13C and 31P nuclear magnetic resonance.
MedLine Citation:
PMID:  6358205     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The structure and metal-binding properties of lipopolysaccharides (LPS) from heptoseless mutants of Escherichia coli were studied by 13C and 31P NMR techniques. Carbon-13 NMR spectra were used to determine the linkages and configurations of the saccharide backbone and the types and locations of fatty acyl groups in E. coli LPS. Resonance assignments for native LPS were made by chemical shift correlation with model compounds, deacylated LPS, lipid A, deacylated lipid A, and fatty acids released from LPS by mild alkaline hydrolysis. The 3-deoxy-D-manno-octulosonate (KDO) disaccharide was tentatively assigned the structure KDO alpha 2 leads to 5KDO alpha 2 leads to. The presence of amide- and ester-linked 3-hydroxy and 3-acyloxy fatty acids in native LPS was confirmed directly from the 13C spectrum and evidence is presented for a labile acyl ester at C-3' (GlcNII) of the lipid A moiety. A significant finding was that the KDO disaccharide is linked to the C-6' position of the lipid A moiety, rather than C-3', as previously reported. The effects of binding Ca2+, Cd2+, Yb3+, Gd3+, and La3+ on the 31P NMR spectrum of LPS indicated that the glycosidic diphosphate moiety participates in a high affinity metal-binding site.
Authors:
S M Strain; S W Fesik; I M Armitage
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  258     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1983 Nov 
Date Detail:
Created Date:  1984-01-07     Completed Date:  1984-01-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  13466-77     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cadmium
Calcium
Carbohydrate Conformation
Carbohydrate Sequence
Escherichia coli / genetics*
Heptoses / genetics*
Lipopolysaccharides* / genetics
Magnetic Resonance Spectroscopy
Metals / metabolism*
Metals, Rare Earth
Mutation*
Grant Support
ID/Acronym/Agency:
AM18778/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Heptoses; 0/Lipopolysaccharides; 0/Metals; 0/Metals, Rare Earth; 7440-43-9/Cadmium; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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