Document Detail

Structure of the lnlB leucine-rich repeats, a domain that triggers host cell invasion by the bacterial pathogen L. monocytogenes.
MedLine Citation:
PMID:  10635330     Owner:  NLM     Status:  MEDLINE    
The L. monocytogenes protein lnlB activates phosphoinositide 3-kinase and induces phagocytosis in several mammalian cell types. The 1.86 A resolution X-ray crystal structure of the leucine-rich repeat domain of lnlB that is both necessary and sufficient to induce phagocytosis is presented here. The structure supports a crucial role for calcium in host cell invasion by L. monocytogenes and supplies a rationale for its function. Calciums are bound to the protein in an unusually exposed manner that suggests that the metals may act as a bridge between lnlB and mammalian cell surface receptors. The structure also identifies surfaces on the curved and elongated molecule that may constitute additional interaction sites in forming a bacterial-mammalian signaling complex.
M Marino; L Braun; P Cossart; P Ghosh
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular cell     Volume:  4     ISSN:  1097-2765     ISO Abbreviation:  Mol. Cell     Publication Date:  1999 Dec 
Date Detail:
Created Date:  2000-01-31     Completed Date:  2000-01-31     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9802571     Medline TA:  Mol Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1063-72     Citation Subset:  IM    
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla 92093-0314, USA.
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MeSH Terms
Amino Acid Sequence
Bacterial Proteins / genetics
Gene Expression Regulation, Bacterial*
Listeria Infections / microbiology*
Listeria monocytogenes / pathogenicity,  physiology*
Membrane Proteins / genetics*
Molecular Sequence Data
Sequence Alignment
Grant Support
Reg. No./Substance:
0/Bacterial Proteins; 0/Membrane Proteins; 0/inlB protein, Listeria monocytogenes; 61-90-5/Leucine

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