Document Detail


Structure, interaction and real-time monitoring of the enzymatic reaction of wild-type APOBEC3G.
MedLine Citation:
PMID:  19153609     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human APOBEC3G exhibits anti-human immunodeficiency virus-1 (HIV-1) activity by deaminating cytidines of the minus strand of HIV-1. Here, we report a solution structure of the C-terminal deaminase domain of wild-type APOBEC3G. The interaction with DNA was examined. Many differences in the interaction were found between the wild type and recently studied mutant APOBEC3Gs. The position of the substrate cytidine, together with that of a DNA chain, in the complex, was deduced. Interestingly, the deamination reaction of APOBEC3G was successfully monitored using NMR signals in real time. Real-time monitoring has revealed that the third cytidine of the d(CCCA) segment is deaminated at an early stage and that then the second one is deaminated at a late stage, the first one not being deaminated at all. This indicates that the deamination is carried out in a strict 3' --> 5' order. Virus infectivity factor (Vif) of HIV-1 counteracts the anti-HIV-1 activity of APOBEC3G. The structure of the N-terminal domain of APOBEC3G, with which Vif interacts, was constructed with homology modelling. The structure implies the mechanism of species-specific sensitivity of APOBEC3G to Vif action.
Authors:
Ayako Furukawa; Takashi Nagata; Akimasa Matsugami; Yuichirou Habu; Ryuichi Sugiyama; Fumiaki Hayashi; Naohiro Kobayashi; Shigeyuki Yokoyama; Hiroshi Takaku; Masato Katahira
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-15
Journal Detail:
Title:  The EMBO journal     Volume:  28     ISSN:  1460-2075     ISO Abbreviation:  EMBO J.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-02-19     Completed Date:  2009-03-26     Revised Date:  2010-09-23    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  England    
Other Details:
Languages:  eng     Pagination:  440-51     Citation Subset:  IM    
Affiliation:
Supramolecular Biology, International Graduate School of Arts and Sciences, Yokohama City University, Yokohama, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Codon
Cytidine / chemistry
Cytidine Deaminase / chemistry*,  metabolism*
DNA, Single-Stranded / chemistry
HIV-1 / metabolism*
Humans
Hydrogen-Ion Concentration
Kinetics
Magnetic Resonance Spectroscopy
Models, Molecular
Molecular Conformation
Mutation
Protein Structure, Tertiary
vif Gene Products, Human Immunodeficiency Virus / metabolism
Chemical
Reg. No./Substance:
0/Codon; 0/DNA, Single-Stranded; 0/vif Gene Products, Human Immunodeficiency Virus; 65-46-3/Cytidine; EC 3.5.4.5/APOBEC3G protein, human; EC 3.5.4.5/Cytidine Deaminase
Comments/Corrections

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