Document Detail

Structure of a glycerol teichoic acid-like O-specific polysaccharide of Proteus vulgaris O12.
MedLine Citation:
PMID:  10651815     Owner:  NLM     Status:  MEDLINE    
A phosphorylated O-specific polysaccharide (O-antigen) was obtained by mild acid degradation of Proteus vulgaris O12 lipopolysaccharide and studied by sugar and methylation analyses, 1H-, 13C- and 31P-NMR spectroscopy, including two-dimensional COSY, TOCSY, NOESY, H-detected 1H, 13C and 1H, 31P heteronuclear multiple-quantum coherence experiments. It was found that the polysaccharide consists of pentasaccharide repeating units connected via a glycerol phosphate group, and has the following structure: where FucNAc is 2-acetamido-2,6-dideoxygalactose and the degree of O-acetylation at position 4 of GalNAc is approximately 25%. Immunochemical studies with P. vulgaris O12 O-antiserum suggested that the lipopolysaccharide studied shares common epitopes with the lipopolysaccharide core of P. vulgaris O8 and with the O-antigens of P. penneri strains 8 and 63.
A V Perepelov; A Torzewska; A S Shashkov; S N Senchenkova; A Rozalski; Y A Knirel
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  267     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-03-08     Completed Date:  2000-03-08     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  788-93     Citation Subset:  IM    
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
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MeSH Terms
Antibodies, Bacterial
Carbohydrate Conformation
Carbohydrate Sequence
Cross Reactions
Glycerol / chemistry
Magnetic Resonance Spectroscopy
Molecular Sequence Data
O Antigens / chemistry*
Proteus / chemistry,  immunology
Proteus vulgaris / chemistry*,  immunology*
Species Specificity
Teichoic Acids / chemistry
Reg. No./Substance:
0/Antibodies, Bacterial; 0/O Antigens; 0/Teichoic Acids; 56-81-5/Glycerol

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