Document Detail

Structure-function relationship of hammerhead ribozymes as probed by 2'-modifications.
MedLine Citation:
PMID:  1841379     Owner:  NLM     Status:  MEDLINE    
Hammerhead ribozymes containing 2'-fluoro- or 2'-aminonucleotides were prepared by automated chemical synthesis. Incorporation of 2'-fluorouridines, 2'-fluorocytidines or 2'-aminouridines did not appreciably decrease catalytic activity. The presence of 2'-aminocytidines, however, reduced the activity about 20-fold. No catalytic activity could be measured for ribozymes in which all adenosines were replaced by the 2'-fluoro analogue in presence of MgCl2. No single position could be found responsible for this loss of activity. In an attempt to construct ribozymes to hydrolyse HIV-RNA in the 5'-LTR region several constructs were tested on synthetic substrate as well as on run-off transcripts of about 1000 nucleotides length.
W A Pieken; D B Olsen; H Aurup; D M Williams; O Heidenreich; F Benseler; F Eckstein
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nucleic acids symposium series     Volume:  -     ISSN:  0261-3166     ISO Abbreviation:  Nucleic Acids Symp. Ser.     Publication Date:  1991  
Date Detail:
Created Date:  1992-11-25     Completed Date:  1992-11-25     Revised Date:  2001-11-13    
Medline Journal Info:
Nlm Unique ID:  8007206     Medline TA:  Nucleic Acids Symp Ser     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  51-3     Citation Subset:  IM; X    
Max-Planck-Institut für Experimentelle Medizin, Göttingen, FRG.
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MeSH Terms
Antiviral Agents / chemical synthesis,  pharmacology
Base Sequence
HIV / drug effects
HIV Long Terminal Repeat
Molecular Sequence Data
Nucleic Acid Conformation
Oligoribonucleotides / chemical synthesis*,  pharmacology
RNA, Catalytic / chemical synthesis*,  chemistry,  metabolism
RNA, Viral / drug effects
Reg. No./Substance:
0/Antiviral Agents; 0/Oligoribonucleotides; 0/RNA, Catalytic; 0/RNA, Viral; 0/Ribonucleotides

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