Document Detail


Structure and function of frozen cells: freezing patterns and post-thaw survival.
MedLine Citation:
PMID:  349159     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Freezing patterns and post-thaw survival of cells varies with different cooling rates. The optimal cooling rates, indicating the highest percentage survival, were different in yeast and red blood cells. A difference of freezing patterns was also noticed in preparations frozen above and below the optimal cooling rate for each cell, namely, cell shrinkage at lower rates and intracellular ice formation at higher rates which showed similar trends in both the cells, even though there was some shifting of the optimum. Ultra-rapid freezing and addition of cryoprotectants are useful ways to minimize ice crystal formation and to cause such ice formations to approach the vitreous state. Ice crystals are hardly detectable in yeast cells as well as in erythrocytes, when these cells are frozen ultra-rapidly in the presence of cryoprotective agents in moderate concentration.
Authors:
T Nei
Related Documents :
20471379 - Investigating cryoinjury using simulations and experiments. 1: tf-1 cells during two-st...
8050269 - The influence of cryopreservation on parameters of energetic metabolism and motility of...
10600259 - Cryopreservation of isolated fish blastomeres: effects of cell stage, cryoprotectant co...
2415599 - A1 and a2 erythrocytes can be distinguished by reagents that do not detect structural d...
20159009 - The relevance of ice crystal formation for the cryopreservation of tissues and organs.
15052419 - Cheese whey: an alternative growth and protective medium for rhizobium loti cells.
23391929 - Application of ldh-release assay to cellular-level evaluation of the toxic potential of...
7145839 - 46,xx/46xy chromosome complement in amniotic fluid cell culture followed by the birth o...
24337849 - Holotransferrin enhances selective anticancer activity of artemisinin against human hep...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of microscopy     Volume:  112     ISSN:  0022-2720     ISO Abbreviation:  J Microsc     Publication Date:  1978 Mar 
Date Detail:
Created Date:  1978-07-15     Completed Date:  1978-07-15     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0204522     Medline TA:  J Microsc     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  197-204     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cell Survival / drug effects
Cryoprotective Agents
Erythrocytes / physiology,  ultrastructure*
Freezing*
Glycerol / pharmacology
Hemolysis / drug effects
Humans
Saccharomyces cerevisiae / physiology,  ultrastructure*
Chemical
Reg. No./Substance:
0/Cryoprotective Agents; 56-81-5/Glycerol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Folate antagonists. 11. Synthesis and antimalarial effects of 6-[(aryloxy- and arylthio-)methyl]-2,4...
Next Document:  Two rapid and simple methods used for the removal of resins from 1.0 micron thick epoxy sections.