Document Detail

Structure and function of frozen cells: freezing patterns and post-thaw survival.
MedLine Citation:
PMID:  349159     Owner:  NLM     Status:  MEDLINE    
Freezing patterns and post-thaw survival of cells varies with different cooling rates. The optimal cooling rates, indicating the highest percentage survival, were different in yeast and red blood cells. A difference of freezing patterns was also noticed in preparations frozen above and below the optimal cooling rate for each cell, namely, cell shrinkage at lower rates and intracellular ice formation at higher rates which showed similar trends in both the cells, even though there was some shifting of the optimum. Ultra-rapid freezing and addition of cryoprotectants are useful ways to minimize ice crystal formation and to cause such ice formations to approach the vitreous state. Ice crystals are hardly detectable in yeast cells as well as in erythrocytes, when these cells are frozen ultra-rapidly in the presence of cryoprotective agents in moderate concentration.
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of microscopy     Volume:  112     ISSN:  0022-2720     ISO Abbreviation:  J Microsc     Publication Date:  1978 Mar 
Date Detail:
Created Date:  1978-07-15     Completed Date:  1978-07-15     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0204522     Medline TA:  J Microsc     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  197-204     Citation Subset:  IM    
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MeSH Terms
Cell Survival / drug effects
Cryoprotective Agents
Erythrocytes / physiology,  ultrastructure*
Glycerol / pharmacology
Hemolysis / drug effects
Saccharomyces cerevisiae / physiology,  ultrastructure*
Reg. No./Substance:
0/Cryoprotective Agents; 56-81-5/Glycerol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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