Document Detail


Structure-function correlations in cryopreserved allograft cardiac valves.
MedLine Citation:
PMID:  7646139     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This communication briefly summarizes a morphologic investigation of explanted cryopreserved heart valves and discusses the findings in the context of ongoing debates regarding modes of failure, cellular viability, durability of the extracellular matrix, and the contribution of immune responses. We studied 20 cryopreserved human heart valve allografts functioning up to 9 years as either orthotopic aortic valves/root replacements or right ventricle-to-pulmonary artery conduits explanted for valve failure, infection, or growth-related conduit or valve stenosis. Implanted valves had progressively severe loss of normal layered structure and were devoid of stainable deep connective tissue cells. Inflammation was minimal. Other late findings included minimal inflammation, mild cuspal hematomas, mural thrombus, and calcification, most prominent in the aortic wall. Transmission electron microscopy of late explants revealed nonviable cells and their debris, and a collagenous skeleton that was largely intact. We conclude that cryopreserved allograft heart valves have minimal, if any, viable cells, but largely retain the original collagen network; preservation of the autolysis-resistant collagenous skeleton likely provides the structural basis of function. Our results also suggest that immune responsiveness has little, if any, impact on late allograft function or degradation.
Authors:
R N Mitchell; R A Jonas; F J Schoen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Annals of thoracic surgery     Volume:  60     ISSN:  0003-4975     ISO Abbreviation:  Ann. Thorac. Surg.     Publication Date:  1995 Aug 
Date Detail:
Created Date:  1995-09-20     Completed Date:  1995-09-20     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  15030100R     Medline TA:  Ann Thorac Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  S108-12; discussion S113     Citation Subset:  AIM; IM    
Affiliation:
Department of Pathology, Brigham and Women's Hospital, Boston, MA 02115, USA.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Cell Survival
Child
Child, Preschool
Cryopreservation*
Extracellular Matrix / pathology
Graft Survival
Heart Valves / pathology,  transplantation*
Humans
Middle Aged
Transplantation Immunology
Transplantation, Homologous / immunology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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