Document Detail

Structure and expression of the rice mitochondrial apocytochrome b gene (cob-1) and pseudogene (cob-2).
MedLine Citation:
PMID:  1282087     Owner:  NLM     Status:  MEDLINE    
Rice mitochondrial DNA contains an intact copy and a pseudogene copy of a apocytochrome b gene (cob-1 and cob-2, respectively). Using primer extension and capping analyses, the transcriptional start site has been mapped; an 11-base motif at the transcription start site closely matches the consensus promoter motifs proposed for maize, wheat and soybean mitochondrial genes. Although both copies are identical in the 5' upstream region and through most of the coding region, only cob-1-specific mRNA is detected on RNA gel-blots. Run-on transcription analysis indicates, however, that both cob-1 and cob-2 mRNAs are synthesized in vivo but less cob-2 is accumulated. At its mapped 3' terminus the cob-1 transcript possesses a sequence that could fold into a double stem-loop structure. The possible roles of a double stem-loop structure in mitochondrial gene expression are discussed.
E K Kaleikau; C P André; V Walbot
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Current genetics     Volume:  22     ISSN:  0172-8083     ISO Abbreviation:  Curr. Genet.     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1993-02-02     Completed Date:  1993-02-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8004904     Medline TA:  Curr Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  463-70     Citation Subset:  IM    
Department of Biological Sciences, Stanford University, CA 94305-5020.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/M81727;  M81728;  M81754;  S72771;  X170064;  X533699;  X65481;  X65482;  Z11730;  Z11731;  Z11733
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MeSH Terms
Apoproteins / genetics*
Base Sequence
Blotting, Northern
Blotting, Southern
Cytochrome b Group / genetics*
Cytochromes b
DNA, Mitochondrial
Gene Expression
Mitochondria / enzymology*
Molecular Sequence Data
Nucleic Acid Hybridization
Oryza sativa / enzymology*
RNA / chemistry
Restriction Mapping
Sequence Homology, Nucleic Acid
Reg. No./Substance:
0/Apoproteins; 0/Cytochrome b Group; 0/DNA, Mitochondrial; 63231-63-0/RNA; 9035-37-4/Cytochromes b

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