Document Detail


Structure and expression of cytochrome f in an Oenothera plastome mutant.
MedLine Citation:
PMID:  2390787     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The chloroplast mutant pm7 is one of a number of mutants derived from the plastome mutator (pm) line of Oenothera hookeri, strain Johansen. Immunoblotting showed that this mutant accumulates a protein that is cross-antigenic with cytochrome f, but five kilodaltons larger than the mature wild-type protein. Since cytochrome f is known to be translated on plastid ribosomes as a precursor with an amino-terminal extension, it is proposed that the unprocessed cytochrome f precursor accumulates in pm7. In addition to this precursor-sized cytochrome f protein, some mature-sized cytochrome f was also found in the mutant plastids. The pm7 mutation is inherited in a non-Mendelian fashion; but no alterations in chloroplast DNA restriction patterns, or differences in DNA sequence in the region encoding cytochrome f, were found in a comparison of the wild-type and pm7 chloroplast DNAs. Although the mutant was capable of synthesizing heme, no covalently-bound heme, normally found associated with mature, functional, cytochrome f was detected in the mutant at sizes expected for the presumed precursor, or for mature cytochrome f. These results indicate that the aberrant accumulation of a precursor-sized cytochrome f in pm7 is not due to a lesion directly in the plastid gene encoding cytochrome f, petA, or to a deficiency in the ability of the mutant plastids to synthesize or accumulate heme.
Authors:
E M Johnson; B B Sears
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Current genetics     Volume:  17     ISSN:  0172-8083     ISO Abbreviation:  Curr. Genet.     Publication Date:  1990 Jun 
Date Detail:
Created Date:  1990-10-04     Completed Date:  1990-10-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8004904     Medline TA:  Curr Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  529-34     Citation Subset:  IM    
Affiliation:
Department of Botany and Plant Pathology, Michigan State University, East Lansing 48824.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Chloroplasts / analysis,  metabolism*
Cytochromes / genetics*
Cytochromes f
Electrophoresis, Polyacrylamide Gel
Genes, Plant
Heme / analysis
Immune Sera
Molecular Sequence Data
Mutation
Plants / genetics*
Restriction Mapping
Chemical
Reg. No./Substance:
0/Cytochromes; 0/Immune Sera; 14875-96-8/Heme; 9035-46-5/Cytochromes f

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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