Document Detail


Structure-efficiency relationship in derivatives of stilbene. Comparison of resveratrol, pinosylvin and pterostilbene.
MedLine Citation:
PMID:  18987580     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: Oxidative stress is related to a number of autoimmune diseases, e.g. rheumatoid arthritis, cancer, etc. The main source of pathologically working reactive oxygen species (ROS) are activated polymorphonuclear leukocytes (PMNL). OBJECTIVE: There are some papers comparing structure - pharmacological efficiency relationship of vegetal substances from the stilbenoid group. We compared the effect of trans-resveratrol, which is well-known by its antioxidative activity, with the effect of pinosylvin and pterostilbene. METHODS: Luminol-enhanced chemiluminescence (CL) was used to study the antioxidative action. The effect was observed in whole blood and in isolated PMNL. The concentrations of substances tested were 0.01-100 microM. Due to the different abilities of luminol and isoluminol to pass through the cell membrane, we studied the effect of the substances tested on intracellular and extracellular ROS. To stimulate the production of ROS we used phorbol-myristate-acetate (PMA), which activates PMNL via protein kinase C. RESULTS: Resveratrol, pinosylvin and pterostilbene inhibited significantly the CL of whole blood and extra- and intracellular CL of isolated PMNL in a dosedependent manner. Depending on different functional groups of the stilbene molecule, resveratrol inhibited CL of whole blood and isolated PMNL, whereas pinosylvin influenced mainly intracellular CL and pterostilbene extracellular CL. CONCLUSION: The presence of different functional groups in the molecules of stilbenoids influence their antioxidative effect. Modification of these functional groups may result in derivatives with required antioxidative properties, targeting mainly extracellular ROS which are responsible for tissue damage during chronic inflammation.
Authors:
Tomas Perecko; Viera Jancinova; Katarina Drabikova; Radomir Nosal; Juraj Harmatha
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuro endocrinology letters     Volume:  29     ISSN:  0172-780X     ISO Abbreviation:  Neuro Endocrinol. Lett.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-11-25     Completed Date:  2009-02-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008373     Medline TA:  Neuro Endocrinol Lett     Country:  Sweden    
Other Details:
Languages:  eng     Pagination:  802-5     Citation Subset:  IM    
Affiliation:
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava, Slovakia. tomas.perecko@savba.sk
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism
Antioxidants / pharmacology*
Cell Survival / drug effects
Dose-Response Relationship, Drug
Humans
Luminescence
Neutrophils / drug effects
Reactive Oxygen Species / metabolism
Stilbenes / pharmacology*
Structure-Activity Relationship
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Reactive Oxygen Species; 0/Stilbenes; 102-61-4/pinosylvin; 501-36-0/resveratrol; 537-42-8/pterostilbene; 56-65-5/Adenosine Triphosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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