Document Detail

Structure of chicken hypothalamic luteinizing hormone-releasing hormone. II. Isolation and characterization.
MedLine Citation:
PMID:  7050119     Owner:  NLM     Status:  MEDLINE    
Avian luteinizing hormone-releasing hormone (LH-RH) has been isolated from 249,000 chicken hypothalami and shown to differ structurally from mammalian hypothalamic LH-RH. Purification was achieved by acetic acid extraction, anti-LH-RH affinity chromatography, and cation exchange and reverse phase high performance liquid chromatography. The isolated peptide eluted as a single peak on reverse phase high performance liquid chromatography. Acid hydrolysis of the peptide yielded integral molar ratios of amino acids and a composition identical with that of mammalian decapeptide LH-RH, except for the presence of an additional glutamic acid residue and the absence of arginine. The isoelectric point of chicken LH-RH (7.3) is consistent with the glutamic acid representing a glutamine residue. We therefore synthesized [Gln8]LH-RH and established that it has chromatographic properties identical with natural chicken LH-RH. These studies indicate that the structure of chicken hypothalamic LH-RH is: pGlu-His-Trp-Ser-Tyr-Gly-Leu-Gln-Pro-Gly-NH2.
J A King; R P Millar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  257     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1982 Sep 
Date Detail:
Created Date:  1982-10-29     Completed Date:  1982-10-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  10729-32     Citation Subset:  IM    
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MeSH Terms
Amino Acids / analysis
Chromatography, Affinity
Chromatography, High Pressure Liquid
Gonadotropin-Releasing Hormone / isolation & purification*
Hypothalamus / analysis*
Peptide Fragments / analysis
Reg. No./Substance:
0/Amino Acids; 0/Peptide Fragments; 33515-09-2/Gonadotropin-Releasing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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