Document Detail


Structure characterization of a fucose-containing exopolysaccharide produced by Enterobacter cloacae Z0206.
MedLine Citation:
PMID:  23218327     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A novel high molecular weight (1.1×10(6)Da) exopolysaccharide (EPS) produced by Enterobacter cloacae Z0206 strain was isolated by column chromatography. Complete hydrolysis of the EPS followed by gas chromatography mass spectrometry (GC-MS) and high performance liquid chromatography (HPLC) analyses showed that the EPS is composed of l-fucose, d-glucose, d-galactose, d-glucuronic acid and pyruvic acid in the approximate molar ratio of 2:1:3:1:1. Partial acid hydrolysis of the purified EPS followed by gel permeation chromatography (GPC) yielded a hexasaccharide. A combination of chemical analysis coupled with mass spectrometry and 1D and 2D NMR spectroscopy applied to the oligosaccharide showed that the EPS comprises a heptasaccharide repeating unit.
Authors:
Fengqin Wang; Hangxian Yang; Yizhen Wang
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Publication Detail:
Type:  Journal Article     Date:  2012-10-12
Journal Detail:
Title:  Carbohydrate polymers     Volume:  92     ISSN:  1879-1344     ISO Abbreviation:  Carbohydr Polym     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8307156     Medline TA:  Carbohydr Polym     Country:  England    
Other Details:
Languages:  eng     Pagination:  503-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
College of Animal Science, Zhejiang University, Key Laboratory of Animal Nutrition and Feed Science, Ministry of Agriculture (East China), Zhejiang Provincial Laboratory of Feed and Animal Nutrition, Hangzhou 310058, China.
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