| Structure characterization of a fucose-containing exopolysaccharide produced by Enterobacter cloacae Z0206. | |
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MedLine Citation:
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PMID: 23218327 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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A novel high molecular weight (1.1×10(6)Da) exopolysaccharide (EPS) produced by Enterobacter cloacae Z0206 strain was isolated by column chromatography. Complete hydrolysis of the EPS followed by gas chromatography mass spectrometry (GC-MS) and high performance liquid chromatography (HPLC) analyses showed that the EPS is composed of l-fucose, d-glucose, d-galactose, d-glucuronic acid and pyruvic acid in the approximate molar ratio of 2:1:3:1:1. Partial acid hydrolysis of the purified EPS followed by gel permeation chromatography (GPC) yielded a hexasaccharide. A combination of chemical analysis coupled with mass spectrometry and 1D and 2D NMR spectroscopy applied to the oligosaccharide showed that the EPS comprises a heptasaccharide repeating unit. |
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Authors:
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Fengqin Wang; Hangxian Yang; Yizhen Wang |
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Publication Detail:
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Type: Journal Article Date: 2012-10-12 |
Journal Detail:
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Title: Carbohydrate polymers Volume: 92 ISSN: 1879-1344 ISO Abbreviation: Carbohydr Polym Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2012-12-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8307156 Medline TA: Carbohydr Polym Country: England |
Other Details:
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Languages: eng Pagination: 503-9 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Elsevier Ltd. All rights reserved. |
Affiliation:
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College of Animal Science, Zhejiang University, Key Laboratory of Animal Nutrition and Feed Science, Ministry of Agriculture (East China), Zhejiang Provincial Laboratory of Feed and Animal Nutrition, Hangzhou 310058, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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