Document Detail

Structure, cellular distribution, antigenicity, and biological functions of Fonsecaea pedrosoi ceramide monohexosides.
MedLine Citation:
PMID:  16299276     Owner:  NLM     Status:  MEDLINE    
Monohexosylceramides (CMHs, or cerebrosides) have been reported as membrane and cell wall constituents of both pathogenic and nonpathogenic fungi, presenting remarkable differences in their ceramide moiety compared to mammalian CMHs. Current evidence suggests that CMHs are involved in fungal differentiation and growth and contribute to host immune response. Here we describe a structural diversity between cerebrosides obtained from different forms of the human pathogen Fonsecaea pedrosoi. The major CMH species produced by conidial forms displayed the same structure previously demonstrated by our group for mycelia, an N-2'-hydroxyhexadecanoyl-1-beta-d-glucopyranosyl-9-methyl-4,8-sphingadienine. However, the major cerebroside species purified from sclerotic cells carries an additional hydroxyl group, bound to its long-chain base. The structural difference between cerebrosides from mycelial and sclerotic cells was apparently not relevant for their antigenicity, since they were both recognized at similar levels by sera from individuals with chromoblastomycosis and a monoclonal antibody to a conserved cerebroside structure. Preincubation of fungal cells with anti-CMH monoclonal antibodies had no effect on the interaction of F. pedrosoi sclerotic cells with murine macrophages. In contrast to what has been described for other fungal species, sclerotic bodies are resistant to the antifungal action of anti-CMH antibodies. Immunofluorescence analysis showed that recognition of sclerotic cells by these antibodies only occurs at cell wall regions in which melanization is not evident. Accordingly, melanin removal with alkali results in an increased reaction of fungal cells with anti-CMH antibodies. Our results indicate that cerebroside expression in F. pedrosoi cells is associated with dimorphism and melanin assembly on the fungal cell wall.
Leonardo Nimrichter; Mariana D Cerqueira; Eduardo A Leitão; Kildare Miranda; Ernesto S Nakayasu; Sandro R Almeida; Igor C Almeida; Celuta S Alviano; Eliana Barreto-Bergter; Marcio L Rodrigues
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Infection and immunity     Volume:  73     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-11-21     Completed Date:  2006-01-06     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7860-8     Citation Subset:  IM    
Instituto de Microbiologia Professor Paulo de Góes, Departamento de Microbiologia Geral, Universidade Federal do Rio de Janeiro Cidade Universitária, Rio de Janeiro, RJ, 21941-590, Brazil.
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MeSH Terms
Antibodies, Fungal / immunology
Antigens, Fungal / chemistry*,  immunology*,  metabolism
Ascomycota / immunology*,  metabolism
Cerebrosides / chemistry*,  immunology*,  metabolism
Lipids / chemistry
Macrophages / immunology
Melanins / metabolism
Grant Support
Reg. No./Substance:
0/Antibodies, Fungal; 0/Antigens, Fungal; 0/Cerebrosides; 0/Lipids; 0/Melanins; 0/ceramide monohexoside

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