Document Detail

Structure of amplified normal and variant dihydrofolate reductase genes in mouse sarcoma S180 cells.
MedLine Citation:
PMID:  6282858     Owner:  NLM     Status:  MEDLINE    
We constructed a gene library from a murine cell line with amplified dihydrofolate reductase (dhfr) genes by inserting random segments of DNA into lambda Ch4A. From this library, the dhfr gene and 30 kilobase pairs of surrounding DNA were cloned, and the restriction map was determined. All of the coding regions were sequenced and show that the gene spans a total of 31 kilobase pairs and has five intervening sequences in the coding portion of the gene. In addition, two classes of variant dhfr genes were found in the amplified line, which were amplified and present at levels of 10 to 30% of the normal dhfr genes. Numerous repeated sequences were located throughout the gene region, some of which share homology with previously defied families of repeats.
G F Crouse; C C Simonsen; R N McEwan; R T Schimke
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  257     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1982 Jul 
Date Detail:
Created Date:  1982-08-26     Completed Date:  1982-08-26     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7887-97     Citation Subset:  IM    
Data Bank Information
Bank Name/Acc. No.:
GENBANK/J00382;  J00383;  J00384;  J00385;  J00386
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MeSH Terms
Base Sequence
DNA Restriction Enzymes
DNA, Recombinant*
Genetic Variation*
Nucleic Acid Hybridization
Repetitive Sequences, Nucleic Acid
Sarcoma 180 / enzymology*
Tetrahydrofolate Dehydrogenase / genetics*
Grant Support
CA 16318/CA/NCI NIH HHS; GM 14931/GM/NIGMS NIH HHS; N01-C0-75380//PHS HHS
Reg. No./Substance:
0/DNA, Recombinant; EC Dehydrogenase; EC 3.1.21.-/DNA Restriction Enzymes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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