| Structure-activity studies of alpha-melanotropin fragments on cAMP production in striatal slices. | |
| | |
MedLine Citation:
|
PMID: 10959000 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In this work, we characterized the active site in the alpha-melanotropin hormone (alpha-MSH) sequence responsible for the enhancement of cAMP production in incubated striatal slices by using different alpha-MSH fragments. We also analyzed the effects of the co-incubation of the SCH23390, a dopaminergic D(1) antagonist, with the MSH fragments, to study the involvement of the D(1) receptor on this induction. A rise was observed in the levels of cAMP after addition of the 6 microM fragments MSH((1-10)), and 0.6 and 6 microM MSH((5-13)); however, the values were lower than those induced by 6 microM alpha-MSH. On the contrary, the addition of MSH((9-13)), MSH((7-11)), or MSH((6-9)) did not affect the cAMP content. The presence of 10 microM SCH23390 blocked the effect of the fragments on cAMP production. We conclude that the biologic activity of alpha-MSH, as observed through the levels of cAMP, declines when the length of its polypeptide chain is shortened, and that the presence of glutamic acid in the molecule, as well as the core sequence, are of importance for fragments' activity. |
| | |
Authors:
|
M Cecilia Cremer; M Silvina Sanchez; M Ester Celis |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Peptides Volume: 21 ISSN: 0196-9781 ISO Abbreviation: Peptides Publication Date: 2000 Jun |
Date Detail:
|
Created Date: 2000-10-19 Completed Date: 2000-10-19 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 8008690 Medline TA: Peptides Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 803-6 Citation Subset: IM |
Affiliation:
|
Laboratorio de Fisiología, Departamento de Farmacología, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, CP 5000, Córdoba, Argentina. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Benzazepines / pharmacology Corpus Striatum / drug effects* Cyclic AMP / biosynthesis* Male Oligopeptides / pharmacology* Peptide Fragments / pharmacology Rats Rats, Wistar Receptors, Dopamine D1 / antagonists & inhibitors Structure-Activity Relationship alpha-MSH / pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Benzazepines; 0/Oligopeptides; 0/Peptide Fragments; 0/Receptors, Dopamine D1; 581-05-5/alpha-MSH; 60-92-4/Cyclic AMP |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Melanocortin signaling is decreased during neurotoxin-induced transient hyperphagia and increased bo...
Next Document: Cathepsin B inhibitory peptides derived from beta-casein.