Document Detail

Structure of an acidic O-specific polysaccharide of the bacterium Providencia alcalifaciens O7.
MedLine Citation:
PMID:  10887286     Owner:  NLM     Status:  MEDLINE    
An acidic O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of the bacterium Providencia alcalifaciens O7 and purified by gel chromatography followed by anion-exchange chromatography. On the basis of full acid hydrolysis, methylation, carboxyl reduction, selective cleavage with anhydrous hydrogen fluoride, and 1H- and 13C-NMR spectroscopy, including two-dimensional 1H,1H homonuclear and H-detected 1H,13C heteronuclear correlation spectroscopy and nuclear Overhauser effect spectroscopy (NOESY), the following structure of the linear tetrasaccharide repeating unit of the polysaccharide was established: [figure], where Rhap2Ac is 2-O-acetylrhamnopyranose.
O V Bystrova; G V Zatonskii; S A Borisova; N A Kocharova; A S Shashkov; Y A Knirel; E V Kholodkova; E S Stanislavskii
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry. Biokhimii͡a     Volume:  65     ISSN:  0006-2979     ISO Abbreviation:  Biochemistry Mosc.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-09-19     Completed Date:  2000-09-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376536     Medline TA:  Biochemistry (Mosc)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  677-84     Citation Subset:  IM    
Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 117913, Russia.
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MeSH Terms
Carbohydrate Conformation
Carbohydrate Sequence
Chromatography, Gas
Hydrogen-Ion Concentration
Magnetic Resonance Spectroscopy
Molecular Sequence Data
O Antigens / chemistry*
Providencia / chemistry*
Reg. No./Substance:
0/O Antigens

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