| Structure of an acidic O-specific polysaccharide of the bacterium Providencia alcalifaciens O7. | |
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MedLine Citation:
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PMID: 10887286 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An acidic O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of the bacterium Providencia alcalifaciens O7 and purified by gel chromatography followed by anion-exchange chromatography. On the basis of full acid hydrolysis, methylation, carboxyl reduction, selective cleavage with anhydrous hydrogen fluoride, and 1H- and 13C-NMR spectroscopy, including two-dimensional 1H,1H homonuclear and H-detected 1H,13C heteronuclear correlation spectroscopy and nuclear Overhauser effect spectroscopy (NOESY), the following structure of the linear tetrasaccharide repeating unit of the polysaccharide was established: [figure], where Rhap2Ac is 2-O-acetylrhamnopyranose. |
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Authors:
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O V Bystrova; G V Zatonskii; S A Borisova; N A Kocharova; A S Shashkov; Y A Knirel; E V Kholodkova; E S Stanislavskii |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemistry. Biokhimii͡a Volume: 65 ISSN: 0006-2979 ISO Abbreviation: Biochemistry Mosc. Publication Date: 2000 Jun |
Date Detail:
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Created Date: 2000-09-19 Completed Date: 2000-09-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376536 Medline TA: Biochemistry (Mosc) Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 677-84 Citation Subset: IM |
Affiliation:
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Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 117913, Russia. knirel@ioc.ac.ru |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carbohydrate Conformation Carbohydrate Sequence Chromatography, Gas Hydrogen-Ion Concentration Magnetic Resonance Spectroscopy Molecular Sequence Data O Antigens / chemistry* Providencia / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/O Antigens |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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