Document Detail


Structure of Trypanosoma brucei flagellum accounts for its bihelical motion.
MedLine Citation:
PMID:  21690369     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Trypanosoma brucei is a parasitic protozoan that causes African sleeping sickness. It contains a flagellum required for locomotion and viability. In addition to a microtubular axoneme, the flagellum contains a crystalline paraflagellar rod (PFR) and connecting proteins. We show here, by cryoelectron tomography, the structure of the flagellum in three bending states. The PFR lattice in straight flagella repeats every 56 nm along the length of the axoneme, matching the spacing of the connecting proteins. During flagellar bending, the PFR crystallographic unit cell lengths remain constant while the interaxial angles vary, similar to a jackscrew. The axoneme drives the expansion and compression of the PFR lattice. We propose that the PFR modifies the in-plane axoneme motion to produce the characteristic trypanosome bihelical motility as captured by high-speed light microscope videography.
Authors:
Alexey Y Koyfman; Michael F Schmid; Ladan Gheiratmand; Caroline J Fu; Htet A Khant; Dandan Huang; Cynthia Y He; Wah Chiu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-06-20
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  108     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-07-06     Completed Date:  2011-09-09     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  11105-8     Citation Subset:  IM    
Affiliation:
National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biophysical Phenomena
Cryoelectron Microscopy
Flagella / chemistry*,  physiology*,  ultrastructure
Humans
Models, Biological
Models, Molecular
Movement / physiology
Protein Conformation
Protozoan Proteins / chemistry,  physiology,  ultrastructure
Trypanosoma brucei brucei / chemistry*,  physiology*,  ultrastructure
Grant Support
ID/Acronym/Agency:
P41RR002250/RR/NCRR NIH HHS; T32 AI07471/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Protozoan Proteins; 0/paraflagellar rod proteins, Trypanosoma brucei
Comments/Corrections

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