Document Detail


Structure of Plasmodium falciparum Thrombospondin-Related Anonymous Protein (TRAP) A domain highlights distinct features in apicomplexan von Willebrand Factor A homologues.
MedLine Citation:
PMID:  23317521     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Thrombospondin-related anonymous protein (TRAP), localized in the micronemes and on the surface of sporozoites of infamous malaria parasite Plasmodium, is a key molecule upon infection of mammalian host hepatocytes and invasion of mosquito salivary glands. TRAP contains two adhesive domains responsible for host cell recognition and invasion, and is known to be essential for infectivity. Here we report high resolution crystal structures of the A domain of Plasmodium falciparum TRAP with and without bound Mg2+. The structure reveals a von Willebrand factor A (vWA)-like fold and a functional metal ion-dependent adhesion site (MIDAS), as well as a potential heparan sulphate binding site. Site-directed mutagenesis and cell attachment assays were used to investigate the functional roles of discovered surface epitopes. The reported structures are the first determined for a complete vWA domain of parasitic origin, highlighting unique features among homologous domains from other proteins characterized hitherto. Some of these are conserved among Plasmodiae exclusively, while others may be common to apicomplexan organisms in general.
Authors:
Tero Pihlajamaa; Tommi Kajander; Juho Knuuti; Kaisa Horkka; Amit Sharma; Perttu Permi
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-15
Journal Detail:
Title:  The Biochemical journal     Volume:  -     ISSN:  1470-8728     ISO Abbreviation:  Biochem. J.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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