| Structure of the O-specific polysaccharide of Proteus vulgaris O4 containing a new component of bacterial polysaccharides, 4,6-dideoxy-4. | |
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MedLine Citation:
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PMID: 11322733 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A high-molecular-mass O-specific polysaccharide was obtained by mild acid degradation of Proteus vulgaris O4 lipopolysaccharide followed by GPC. The polysaccharide was studied by chemical methods along with 1H and 13C NMR spectroscopy, including two-dimensional COSY, TOCSY, NOESY, H-detected 1H,13C HMQC, and 1H,13C HMBC experiments. Solvolysis of the polysaccharide with trifluoromethanesulfonic (triflic) acid resulted in a GlcpA-(1 --> 3)-GlcNAc disaccharide and a novel amino sugar derivative, 4,6-dideoxy-4-[N-[(R)-3-hydroxybutyryl]-L-alanyl]amino-D-glucose [Qui4N(HbAla)]. On the basis of the data obtained, the following structure of the tetrasaccharide repeating unit of the O-specific polysaccharide was established: --> 4)-beta-D-GlcpA-(1 --> 3)-beta-D-GlcpNAc-(1 --> 2)-beta-D-Quip4N(HbAla)-(1 --> 3)-alpha-D-Galp-(1 -->. This structure is unique among the O-specific polysaccharides, which is in accordance with classification of the strain studied in a separate Proteus serogroup. |
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Authors:
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A V Perepelov; D Babicka; S N Senchenkova; A S Shashkov; H Moll; A Rozalski; U Zähringer; Y A Knirel |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Carbohydrate research Volume: 331 ISSN: 0008-6215 ISO Abbreviation: Carbohydr. Res. Publication Date: 2001 Mar |
Date Detail:
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Created Date: 2001-04-26 Completed Date: 2001-12-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0043535 Medline TA: Carbohydr Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 195-202 Citation Subset: IM |
Affiliation:
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N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbohydrate Conformation Carbohydrate Sequence Chromatography, Gas Enzyme-Linked Immunosorbent Assay Magnetic Resonance Spectroscopy Molecular Sequence Data Molecular Structure O Antigens / chemistry* Polysaccharides, Bacterial / chemistry* Proteus vulgaris / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/O Antigens; 0/Polysaccharides, Bacterial |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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