Document Detail

Structure of the O-specific polysaccharide of Proteus vulgaris O4 containing a new component of bacterial polysaccharides, 4,6-dideoxy-4.
MedLine Citation:
PMID:  11322733     Owner:  NLM     Status:  MEDLINE    
A high-molecular-mass O-specific polysaccharide was obtained by mild acid degradation of Proteus vulgaris O4 lipopolysaccharide followed by GPC. The polysaccharide was studied by chemical methods along with 1H and 13C NMR spectroscopy, including two-dimensional COSY, TOCSY, NOESY, H-detected 1H,13C HMQC, and 1H,13C HMBC experiments. Solvolysis of the polysaccharide with trifluoromethanesulfonic (triflic) acid resulted in a GlcpA-(1 --> 3)-GlcNAc disaccharide and a novel amino sugar derivative, 4,6-dideoxy-4-[N-[(R)-3-hydroxybutyryl]-L-alanyl]amino-D-glucose [Qui4N(HbAla)]. On the basis of the data obtained, the following structure of the tetrasaccharide repeating unit of the O-specific polysaccharide was established: --> 4)-beta-D-GlcpA-(1 --> 3)-beta-D-GlcpNAc-(1 --> 2)-beta-D-Quip4N(HbAla)-(1 --> 3)-alpha-D-Galp-(1 -->. This structure is unique among the O-specific polysaccharides, which is in accordance with classification of the strain studied in a separate Proteus serogroup.
A V Perepelov; D Babicka; S N Senchenkova; A S Shashkov; H Moll; A Rozalski; U Zähringer; Y A Knirel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Carbohydrate research     Volume:  331     ISSN:  0008-6215     ISO Abbreviation:  Carbohydr. Res.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-04-26     Completed Date:  2001-12-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  195-202     Citation Subset:  IM    
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow.
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MeSH Terms
Carbohydrate Conformation
Carbohydrate Sequence
Chromatography, Gas
Enzyme-Linked Immunosorbent Assay
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Molecular Structure
O Antigens / chemistry*
Polysaccharides, Bacterial / chemistry*
Proteus vulgaris / chemistry*
Reg. No./Substance:
0/O Antigens; 0/Polysaccharides, Bacterial

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