Document Detail


Structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O12.
MedLine Citation:
PMID:  20471004     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O12. Its structure was studied by sugar analysis using GLC of the alditol acetates and (S)-2-octyl glycosides, methylation analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy, including 2D (1)H-(1)H COSY, TOCSY, ROESY, (1)H-(13)C HSQC, and HMBC experiments. It was found that the polymer is a neutral heteropolysaccharide and has a branched heptasaccharide repeating unit with the following structure:
Authors:
Angelina A Parkhomchuk; Nina A Kocharova; Magdalena Białczak-Kokot; Alexander S Shashkov; Alexander O Chizhov; Yuriy A Knirel; Antoni Rozalski
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-10
Journal Detail:
Title:  Carbohydrate research     Volume:  345     ISSN:  1873-426X     ISO Abbreviation:  Carbohydr. Res.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-10     Completed Date:  2010-09-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1235-9     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Elsevier Ltd. All rights reserved.
Affiliation:
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation. lina_chuk@mail.ru
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MeSH Terms
Descriptor/Qualifier:
Carbohydrate Sequence
Magnetic Resonance Spectroscopy
Mass Spectrometry
Molecular Sequence Data
O Antigens / chemistry*,  isolation & purification
Providencia / chemistry*
Chemical
Reg. No./Substance:
0/O Antigens

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