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Structure of the O-polysaccharide of Providencia alcalifaciens O3 containing 3,6-dideoxy-3-formamido-d-glucose and d-galacturonamide.
MedLine Citation:
PMID:  22960211     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Mild acid degradation of the lipopolysaccharide (LPS) of Providencia alcalifaciens O3 followed by GPC on Sephadex G-50 and anion-exchange chromatography on DEAE-Trisacryl M afforded neutral and acidic polysaccharides, LPS core oligosaccharide, and an oligosaccharide composed of one repeat of the neutral polysaccharide (O-unit) linked to the LPS core. The following structure of the pentasaccharide O-unit was established by sugar and methylation analyses, 2D (1)H and (13)C NMR spectroscopy and ESI MS: where Qui3NFo stands for 3,6-dideoxy-3-formamidoglucose and GalAN for galacturonamide. The LPS core is represented by the Glc(3)Gal(1)GalA(1)Hep(3)Kdo(1)Ara4N(1)P(3)EtN(2) glycoform reported earlier for P. alcalifaciens O9, O34, and O19. The acidic polysaccharide had the same peptidoglycan-like structure as a polysaccharide isolated earlier from P. alcalifaciens O24, O38, and O45, and, most likely, represents bacterial capsule material.
Authors:
Olga G Ovchinnikova; Anna N Kondakova; Nina A Kocharova; Alexander S Shashkov; Yuriy A Knirel; Antoni Rozalski
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-8
Journal Detail:
Title:  Carbohydrate research     Volume:  361C     ISSN:  1873-426X     ISO Abbreviation:  Carbohydr. Res.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-9-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  27-32     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia.
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