| Structure of the O-antigen and characterization of the O-antigen gene cluster of Escherichia coli O108 containing 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-D-galacto-non-2-ulosonic (8-epilegionaminic) acid. | |
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MedLine Citation:
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PMID: 20331420 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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On mild acid degradation of the lipopolysaccharide of Escherichia coli O108, the O-polysaccharide was isolated and studied by sugar analysis and one- and two-dimensional 1H- and 13C-NMR spectroscopy. The polysaccharide was found to contain an unusual higher sugar, 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-d-galacto-non-2-ulosonic acid (di-N-acetyl-8-epilegionaminic acid, 8eLeg5Ac7Ac). The following structure of the tetrasaccharide repeating unit of the polysaccharide was established: -->4)-alpha-8eLegp5Ac7Ac-(2-->6)-alpha-D-Galp-(1-->3)-alpha-L-FucpNAc-(1-->3)-alpha-D-GlcpNAc-(1-->. Functions of the E. coli O108 antigen biosynthetic genes, including seven putative genes for synthesis of 8eLeg5Ac7Ac, were assigned by sequencing the O-antigen gene cluster along with comparison with gene databases and known biosynthetic pathways for related nonulosonic acids. |
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Authors:
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A V Perepelov; Bin Liu; S N Senchenkova; A S Shashkov; S D Shevelev; Lu Feng; Lei Wang; Y A Knirel |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemistry. Biokhimii?a Volume: 75 ISSN: 1608-3040 ISO Abbreviation: Biochemistry Mosc. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2010-03-24 Completed Date: 2010-06-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376536 Medline TA: Biochemistry (Mosc) Country: United States |
Other Details:
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Languages: eng Pagination: 19-24 Citation Subset: IM |
Affiliation:
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Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 119991, Russia. perepel@ioc.ac.ru |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carbohydrate Sequence Databases, Genetic Escherichia coli / chemistry*, genetics Genes, Bacterial Magnetic Resonance Spectroscopy Molecular Sequence Data Multigene Family O Antigens / chemistry*, isolation & purification Sialic Acids / biosynthesis*, chemistry |
| Chemical | |
Reg. No./Substance:
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0/O Antigens; 0/Sialic Acids; 112154-63-9/5,7-diacetamido-3,5,7,9-tetradeoxy-glycerogalacto-nonulosonic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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