Document Detail

Structure and Notch receptor binding of the tandem WWE domain of Deltex.
MedLine Citation:
PMID:  16271883     Owner:  NLM     Status:  MEDLINE    
Deltex is a cytosolic effector of Notch signaling thought to bind through its N-terminal domain to the Notch receptor. Here we report the structure of the Drosophila Deltex N-terminal domain, which contains two tandem WWE sequence repeats. The WWE repeats, which adopt a novel fold, are related by an approximate two-fold axis of rotation. Although the WWE repeats are structurally distinct, they interact extensively and form a deep cleft at their junction that appears well suited for ligand binding. The two repeats are thermodynamically coupled; this coupling is mediated in part by a conserved segment that is immediately C-terminal to the second WWE domain. We demonstrate that although the Deltex WWE tandem is monomeric in solution, it forms a heterodimer with the ankyrin domain of the Notch receptor. These results provide structural and functional insight into how Deltex modulates Notch signaling, and how WWE modules recognize targets for ubiquitination.
Mark E Zweifel; Daniel J Leahy; Doug Barrick
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Structure (London, England : 1993)     Volume:  13     ISSN:  0969-2126     ISO Abbreviation:  Structure     Publication Date:  2005 Nov 
Date Detail:
Created Date:  2005-11-07     Completed Date:  2008-07-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101087697     Medline TA:  Structure     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1599-611     Citation Subset:  IM    
T.C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, Maryland 21218, USA.
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MeSH Terms
Amino Acid Sequence
Crystallography, X-Ray
Drosophila Proteins / chemistry*,  genetics,  metabolism*
Drosophila melanogaster / metabolism
Membrane Proteins / chemistry*,  genetics,  metabolism*
Molecular Sequence Data
Protein Binding
Protein Structure, Secondary
Protein Structure, Tertiary
Receptors, Notch / metabolism*
Repetitive Sequences, Amino Acid*
Tandem Repeat Sequences*
Grant Support
Reg. No./Substance:
0/Drosophila Proteins; 0/Membrane Proteins; 0/Receptors, Notch; 0/deltex protein, Drosophila; 0/notch protein, Drosophila
Comment In:
Structure. 2005 Nov;13(11):1579-80   [PMID:  16271878 ]

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