Document Detail

The Structure of Human Tripeptidyl Peptidase II as Determined by a Hybrid Approach.
MedLine Citation:
PMID:  22483107     Owner:  NLM     Status:  In-Data-Review    
Tripeptidyl-peptidase II (TPPII) is a high molecular mass (∼5 MDa) serine protease, which is thought to act downstream of the 26S proteasome, cleaving peptides released by the latter. Here, the structure of human TPPII (HsTPPII) has been determined to subnanometer resolution by cryoelectron microscopy and single-particle analysis. The complex is built from two strands forming a quasihelical structure harboring a complex system of inner cavities. HsTPPII particles exhibit some polymorphism resulting in complexes consisting of nine or of eight dimers per strand. To obtain deeper insights into the architecture and function of HsTPPII, we have created a pseudoatomic structure of the HsTPPII spindle using a comparative model of HsTPPII dimers and molecular dynamics flexible fitting. Analyses of the resulting hybrid structure of the HsTPPII holocomplex provide new insights into the mechanism of maturation and activation.
Anne-Marie Schönegge; Elizabeth Villa; Friedrich Förster; Reiner Hegerl; Jürgen Peters; Wolfgang Baumeister; Beate Rockel
Related Documents :
21140397 - Synthesis and supramolecular self-assembly of coil-rod-coil molecules: the relationship...
16041137 - Koshikamide a2, a cytotoxic linear undecapeptide isolated from a marine sponge of theon...
22771617 - Host-defense peptides of australian anurans. part 2. structure, activity, mechanism of ...
22607407 - Solution structure of an antibody-bound hiv-1(iiib) v3 peptide: a cis proline turn link...
8735587 - Alpha-fetoprotein derived synthetic peptides: assay of an estrogen-modifying regulatory...
11036197 - Caspase-3 activation by beta-amyloid and prion protein peptides is independent from the...
Publication Detail:
Type:  Journal Article     Date:  2012-04-03
Journal Detail:
Title:  Structure (London, England : 1993)     Volume:  20     ISSN:  1878-4186     ISO Abbreviation:  Structure     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-04-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101087697     Medline TA:  Structure     Country:  United States    
Other Details:
Languages:  eng     Pagination:  593-603     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Fabs enable single particle cryoEM studies of small proteins.
Next Document:  Solution structure analysis of the HPV16 E6 oncoprotein reveals a self-association mechanism require...