| Structure of an acidic O-specific polysaccharide of the marine bacterium Pseudoalteromonas agarivorans KMM 232 (R-form). | |
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MedLine Citation:
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PMID: 20632942 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An acidic O-specific polysaccharide containing L-rhamnose, 2-acetamido-2-deoxy-D-galactose, 2,6-dideoxy-2-(N-acetyl-L-threonine)amino-D-galactose, and 2-acetamido-2-deoxy-D-mannuronic acid was obtained by mild acid degradation of the lipopolysaccharide of the marine bacterium Pseudoalteromonas agarivorans KMM 232 (R-form) followed by gel-permeation chromatography. The polysaccharide was subjected to Smith degradation to give a modified polysaccharide with trisaccharide repeating unit containing L-threonine. The initial and modified polysaccharides were studied by sugar analysis and 1H- and 13C-NMR spectroscopy, including COSY, TOCSY, ROESY, and HSQC experiments, and the structure of the branched tetrasaccharide repeating unit of the polysaccharide was established. |
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Authors:
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N A Komandrova; V V Isakov; S V Tomshich; L A Romanenko; A V Perepelov; A S Shashkov |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemistry. Biokhimii͡a Volume: 75 ISSN: 1608-3040 ISO Abbreviation: Biochemistry Mosc. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-07-16 Completed Date: 2010-11-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376536 Medline TA: Biochemistry (Mosc) Country: United States |
Other Details:
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Languages: eng Pagination: 623-8 Citation Subset: IM |
Affiliation:
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Pacific Institute of Bioorganic Chemistry, Far East Division, Russian Academy of Sciences, Vladivostok, 690022, Russia. komand@piboc.dvo.ru |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carbohydrate Sequence Chromatography, Gel Magnetic Resonance Spectroscopy Molecular Sequence Data O Antigens / chemistry*, isolation & purification Pseudoalteromonas / chemistry* Threonine / chemistry |
| Chemical | |
Reg. No./Substance:
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0/O Antigens; 72-19-5/Threonine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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