Document Detail

Structural study of a highly O-acetylated core of Legionella pneumophila serogroup 1 lipopolysaccharide.
MedLine Citation:
PMID:  8938377     Owner:  NLM     Status:  MEDLINE    
A core oligosaccharide was obtained after mild acid degradation of Legionella pneumophila serogroup 1 lipopolysaccharide (LPS). On the basis of chemical, GLC-MS, 1H, and 13C NMR spectroscopic data, it was found that the oligosaccharide obtained is a highly O-acetylated heptasaccharide having the following structure: [formula: see text] where Kdo is 3-deoxy-D-manno-octulosonic acid and QuiNAc is 2-acetamido-2,6-dideoxyglucose. In the LPS, the O-specific polysaccharide chain is linked to position 3 of the terminal rhamnosyl group and is cleaved during degradation of the LPS. The degradation also induced partial migration and partial removal of the O-acetyl group from the terminal rhamnosyl group which, together with the occurrence of the reducing Kdo residue in multiple forms, contributes to the heterogeneity of the isolated core oligosaccharide. No such highly O-acetylated core oligosaccharide has been reported so far for LPS of Gram-negative bacteria.
Y A Knirel; H Moll; U Zähringer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Carbohydrate research     Volume:  293     ISSN:  0008-6215     ISO Abbreviation:  Carbohydr. Res.     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1997-01-30     Completed Date:  1997-01-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  223-34     Citation Subset:  IM    
Forschungszentrum Borstel, Zentrum für Medizin und Biowissenschaften, Germany.
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MeSH Terms
Carbohydrate Conformation
Carbohydrate Sequence
Chromatography, Gel
Electrophoresis, Polyacrylamide Gel
Gas Chromatography-Mass Spectrometry
Legionella pneumophila / chemistry*
Lipopolysaccharides / chemistry*
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Monosaccharides / analysis,  chemistry
Oligosaccharides / chemistry*
Reg. No./Substance:
0/Lipopolysaccharides; 0/Monosaccharides; 0/Oligosaccharides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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