| Structural studies of glycans isolated from rat plasma hemopexin. | |
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MedLine Citation:
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PMID: 6405810 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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After exhaustive pronase digestion, purification by gel filtration and affinity chromatography on concanavalin A, three glycopeptide fractions were obtained from rat hemopexin. Two fractions (I and II) were concanavalin A non-reactive and one (III) was concanavalin A reactive. On the basis of carbohydrate composition, methylation analysis and proton nuclear magnetic resonance spectroscopy, the primary structure of the glycan in fraction III is proposed as being a mixture of mono- and di-sialo-diantennae of the N-glycosidic, N- acetyllactosamine type. Hydrazinolysis of glycopeptides not binding to concanavalin A yielded mixtures of oligosaccharides for both fractions. These oligosaccharides were separated by HPLC; the molar composition of each of them is given. These data suggest that rat hemopexin contains, among others, a diantennary structure bearing three sialic acid residues. |
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Authors:
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N Bernard; C Lombart; G Strecker; J Montreuil; H Van Halbeek; J F Vliegenthart |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochimie Volume: 65 ISSN: 0300-9084 ISO Abbreviation: Biochimie Publication Date: 1983 Mar |
Date Detail:
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Created Date: 1983-07-08 Completed Date: 1983-07-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1264604 Medline TA: Biochimie Country: FRANCE |
Other Details:
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Languages: eng Pagination: 185-92 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbohydrates / analysis Chromatography, High Pressure Liquid Hemopexin / analysis* Magnetic Resonance Spectroscopy Polysaccharides / analysis* Pronase / metabolism Rats |
| Chemical | |
Reg. No./Substance:
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0/Carbohydrates; 0/Polysaccharides; 9013-71-2/Hemopexin; EC 3.4.24.-/Pronase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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