| Structural studies of cytochrome reductase. Subunit topography determined by electron microscopy of membrane crystals of a subcomplex. | |
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MedLine Citation:
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PMID: 6302289 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ubiquinol: cytochrome c reductase was isolated from Neurospora mitochondria as a protein-detergent complex and dissociated by mild salt treatment. Three parts were obtained and characterized. Firstly, a complex containing the subunits III (cytochrome b), IV (cytochrome c1), VI, VII, VIII and IX; secondly, a complex containing the subunits I and II; and thirdly, the single subunit V (iron-sulphur subunit). Membrane crystals were prepared from the cytochrome bc1 subunit complex and by combining tilted electron microscopic views of the crystals, a low-resolution three-dimensional structure was calculated. This structure was compared to that of the whole cytochrome reductase (previously determined by electron microscopy of membrane crystals). Protein density absent from the structure of the subunit complex was then attributed to the missing subunits according to their size and shape and their association with the phospholipid bilayer. |
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Authors:
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B Karlsson; S Hovmöller; H Weiss; K Leonard |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of molecular biology Volume: 165 ISSN: 0022-2836 ISO Abbreviation: J. Mol. Biol. Publication Date: 1983 Apr |
Date Detail:
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Created Date: 1983-06-23 Completed Date: 1983-06-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985088R Medline TA: J Mol Biol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 287-302 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Crystallization Electron Transport Complex III Lipid Bilayers Macromolecular Substances Microscopy, Electron Mitochondria / enzymology Models, Molecular Molecular Weight Multienzyme Complexes* NADH, NADPH Oxidoreductases* Neurospora / enzymology Phospholipids Quinone Reductases* |
| Chemical | |
Reg. No./Substance:
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0/Lipid Bilayers; 0/Macromolecular Substances; 0/Multienzyme Complexes; 0/Phospholipids; EC 1.10.2.2/Electron Transport Complex III; EC 1.6.-/NADH, NADPH Oxidoreductases; EC 1.6.99.-/Quinone Reductases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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