Document Detail


Structural studies of cytochrome reductase. Subunit topography determined by electron microscopy of membrane crystals of a subcomplex.
MedLine Citation:
PMID:  6302289     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ubiquinol: cytochrome c reductase was isolated from Neurospora mitochondria as a protein-detergent complex and dissociated by mild salt treatment. Three parts were obtained and characterized. Firstly, a complex containing the subunits III (cytochrome b), IV (cytochrome c1), VI, VII, VIII and IX; secondly, a complex containing the subunits I and II; and thirdly, the single subunit V (iron-sulphur subunit). Membrane crystals were prepared from the cytochrome bc1 subunit complex and by combining tilted electron microscopic views of the crystals, a low-resolution three-dimensional structure was calculated. This structure was compared to that of the whole cytochrome reductase (previously determined by electron microscopy of membrane crystals). Protein density absent from the structure of the subunit complex was then attributed to the missing subunits according to their size and shape and their association with the phospholipid bilayer.
Authors:
B Karlsson; S Hovmöller; H Weiss; K Leonard
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of molecular biology     Volume:  165     ISSN:  0022-2836     ISO Abbreviation:  J. Mol. Biol.     Publication Date:  1983 Apr 
Date Detail:
Created Date:  1983-06-23     Completed Date:  1983-06-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985088R     Medline TA:  J Mol Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  287-302     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Crystallization
Electron Transport Complex III
Lipid Bilayers
Macromolecular Substances
Microscopy, Electron
Mitochondria / enzymology
Models, Molecular
Molecular Weight
Multienzyme Complexes*
NADH, NADPH Oxidoreductases*
Neurospora / enzymology
Phospholipids
Quinone Reductases*
Chemical
Reg. No./Substance:
0/Lipid Bilayers; 0/Macromolecular Substances; 0/Multienzyme Complexes; 0/Phospholipids; EC 1.10.2.2/Electron Transport Complex III; EC 1.6.-/NADH, NADPH Oxidoreductases; EC 1.6.99.-/Quinone Reductases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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