| Structural studies on the carbohydrate moieties of rat liver cathepsins B and H. | |
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MedLine Citation:
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PMID: 4019443 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cathepsins B and H from rat liver contain one asparagine-linked sugar chain in each molecule. The sugar chains were liberated from the polypeptide portions by hydrazinolysis followed by N-acetylation and NaB3H4 reduction. Paper electrophoresis of the radioactive oligosaccharide fractions revealed that they were mixtures of neutral oligosaccharides only. After fractionation by gel filtration the structure of each oligosaccharide was studied by sequential exoglycosidase digestion in combination with methylation analysis. The sugar chain of cathepsin H was a high mannose type oligosaccharide which varied in size from 5 to 9 mannose residues; on the other hand the major oligosaccharide of cathepsin B was a tetrasaccharide whose structure was Manalpha 1----6Manbeta 1----4GlcNAcbeta 1----4GlcNAc. |
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Authors:
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T Taniguchi; T Mizuochi; T Towatari; N Katunuma; A Kobata |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biochemistry Volume: 97 ISSN: 0021-924X ISO Abbreviation: J. Biochem. Publication Date: 1985 Mar |
Date Detail:
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Created Date: 1985-09-04 Completed Date: 1985-09-04 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0376600 Medline TA: J Biochem Country: JAPAN |
Other Details:
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Languages: eng Pagination: 973-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbohydrates / analysis* Cathepsin B Cathepsin H Cathepsins / isolation & purification* Chemical Phenomena Chemistry Chromatography, Gel Cysteine Endopeptidases* Liver / enzymology* Rats |
| Chemical | |
Reg. No./Substance:
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0/Carbohydrates; EC 3.4.-/Cathepsins; EC 3.4.22.-/Cysteine Endopeptidases; EC 3.4.22.1/Cathepsin B; EC 3.4.22.16/Cathepsin H; EC 3.4.22.16/Ctsh protein, rat |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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