Document Detail

Structural studies of apoptosis and ion transport regulatory proteins in membranes.
MedLine Citation:
PMID:  14745797     Owner:  NLM     Status:  MEDLINE    
Solid-state NMR spectroscopy is being used to determine the structures of membrane proteins involved in the regulation of apoptosis and ion transport. The Bcl-2 family includes pro- and anti-apoptotic proteins that play a major regulatory role in mitochondrion-dependent apoptosis or programmed cell death. The NMR data obtained for (15)N-labeled anti-apoptotic Bcl-xL in lipid bilayers are consistent with membrane association through insertion of the two central hydrophobic alpha-helices that are also required for channel formation and cytoprotective activity. The FXYD family proteins regulate ion flux across membranes, through interaction with the Na(+), K(+)-ATPase, in tissues that perform fluid and solute transport or that are electrically excitable. We have expressed and purified three FXYD family members, Mat8 (mammary tumor protein), CHIF (channel-inducing factor) and PLM (phospholemman), for structure determination by NMR in lipids. The solid-state NMR spectra of Bcl-2 and FXYD proteins, in uniaxially oriented lipid bilayers, give the first view of their membrane-associated architectures.
Carla M Franzin; Jungyuen Choi; Dayong Zhai; John C Reed; Francesca M Marassi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Magnetic resonance in chemistry : MRC     Volume:  42     ISSN:  0749-1581     ISO Abbreviation:  Magn Reson Chem     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-07-02     Completed Date:  2005-03-22     Revised Date:  2014-09-16    
Medline Journal Info:
Nlm Unique ID:  9882600     Medline TA:  Magn Reson Chem     Country:  England    
Other Details:
Languages:  eng     Pagination:  172-9     Citation Subset:  IM    
Copyright Information:
Copyright 2004 John Wiley & Sons, Ltd.
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MeSH Terms
Amino Acid Sequence
Apoptosis / physiology*
Cloning, Molecular
Conserved Sequence
Lipid Bilayers*
Magnetic Resonance Spectroscopy / methods
Membrane Proteins / chemistry*
Models, Molecular
Neoplasm Proteins
Protein Conformation
Protein Structure, Secondary
Proto-Oncogene Proteins c-bcl-2 / chemistry*
Recombinant Proteins / chemistry,  isolation & purification
Grant Support
R01 CA082864/CA/NCI NIH HHS; R01 CA082864-05/CA/NCI NIH HHS; R01 GM065374/GM/NIGMS NIH HHS; R01 GM065374-04/GM/NIGMS NIH HHS; R01CA82864/CA/NCI NIH HHS; R01GM60554/GM/NIGMS NIH HHS
Reg. No./Substance:
0/FXYD3 protein, human; 0/Lipid Bilayers; 0/Membrane Proteins; 0/Neoplasm Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/Recombinant Proteins

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