Document Detail


Structural segregation of gut microbiota between colorectal cancer patients and healthy volunteers.
MedLine Citation:
PMID:  21850056     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Despite a long-suspected role in the development of human colorectal cancer (CRC), the composition of gut microbiota in CRC patients has not been adequately described. In this study, fecal bacterial diversity in CRC patients (n=46) and healthy volunteers (n=56) were profiled by 454 pyrosequencing of the V3 region of the 16S ribosomal RNA gene. Both principal component analysis and UniFrac analysis showed structural segregation between the two populations. Forty-eight operational taxonomic units (OTUs) were identified by redundancy analysis as key variables significantly associated with the structural difference. One OTU closely related to Bacteroides fragilis was enriched in the gut microbiota of CRC patients, whereas three OTUs related to Bacteroides vulgatus and Bacteroides uniformis were enriched in that of healthy volunteers. A total of 11 OTUs belonging to the genera Enterococcus, Escherichia/Shigella, Klebsiella, Streptococcus and Peptostreptococcus were significantly more abundant in the gut microbiota of CRC patients, and 5 OTUs belonging to the genus Roseburia and other butyrate-producing bacteria of the family Lachnospiraceae were less abundant. Real-time quantitative PCR further validated the significant reduction of butyrate-producing bacteria in the gut microbiota of CRC patients by measuring the copy numbers of butyryl-coenzyme A CoA transferase genes (Mann-Whitney test, P<0.01). Reduction of butyrate producers and increase of opportunistic pathogens may constitute a major structural imbalance of gut microbiota in CRC patients.
Authors:
Tingting Wang; Guoxiang Cai; Yunping Qiu; Na Fei; Menghui Zhang; Xiaoyan Pang; Wei Jia; Sanjun Cai; Liping Zhao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-08-18
Journal Detail:
Title:  The ISME journal     Volume:  6     ISSN:  1751-7370     ISO Abbreviation:  ISME J     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-01-17     Completed Date:  2012-06-05     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  101301086     Medline TA:  ISME J     Country:  England    
Other Details:
Languages:  eng     Pagination:  320-9     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
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MeSH Terms
Descriptor/Qualifier:
Acyl Coenzyme A / genetics
Adult
Aged
Bacteria / classification,  genetics
Bacterial Physiological Phenomena*
Biodiversity*
Coenzyme A-Transferases / genetics
Colorectal Neoplasms / microbiology*
Feces / microbiology
Female
Gastrointestinal Tract / chemistry,  microbiology*
Humans
Male
Metagenome*
Middle Aged
Principal Component Analysis
RNA, Ribosomal, 16S / genetics
Reproducibility of Results
Chemical
Reg. No./Substance:
0/Acyl Coenzyme A; 0/RNA, Ribosomal, 16S; 2140-48-9/butyryl-coenzyme A; EC 2.8.3.-/Coenzyme A-Transferases
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