Document Detail

Structural role of the secondary active domain of HIV-2 NCp8 in multi-functionality.
MedLine Citation:
PMID:  17511966     Owner:  NLM     Status:  MEDLINE    
Nucleocapsid protein of HIV, containing two CCHC-type zinc fingers connected by a linker, is a multi-functional protein involved in many critical steps of the HIV life cycle. Several in vitro investigations demonstrated that the reactivities of the first zinc finger flanked by the linker of HIV-1 NCp7 and HIV-2 NCp8 were essential for binding to viral RNA, however, that of the second zinc finger flanked by the linker of NCp7 was very weak and non-specific, whereas the part of NCp8 called NCp8-f2, interacted strongly and specifically with viral RNA. In this study, the three-dimensional structure of NCp8-f2 was determined for the first time. Furthermore, we established that NCp8-f2 specifically binds to the stem-loop SD in viral RNA, and that the hydrophobic cleft and the basic residues close to the cleft were essential for specific binding to SD. We discuss the functional significance of NCp8-f2 for NCp8 being a multi-functional protein.
Takashi Matsui; Yoshio Kodera; Emi Miyauchi; Hidekazu Tanaka; Hiroshi Endoh; Hiroyoshi Komatsu; Takeshi Tanaka; Toshiyuki Kohno; Tadakazu Maeda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-05-04
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  358     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-05-29     Completed Date:  2007-07-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  673-8     Citation Subset:  IM    
Department of Physics, School of Science, Kitasato University, 1-15-1 Kitasato, Sagamihara, Kanagawa 228-8555, Japan.
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MeSH Terms
Amino Acid Sequence
Binding Sites
Capsid Proteins / chemistry*,  physiology*
Magnetic Resonance Spectroscopy
Molecular Conformation
Molecular Sequence Data
Nucleic Acid Conformation
Peptides / chemistry
Protein Binding
Protein Conformation
Protein Structure, Tertiary
RNA / chemistry
RNA-Binding Proteins / chemistry*,  physiology*
Sequence Homology, Amino Acid
Structure-Activity Relationship
Zinc Fingers
Reg. No./Substance:
0/Capsid Proteins; 0/Peptides; 0/RNA-Binding Proteins; 0/nucleocapsid protein 8, HIV-2; 63231-63-0/RNA

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