Document Detail

Structural proteins and glycoproteins of infectious bronchitis virus particles labeled during growth in chick embryo cells.
MedLine Citation:
PMID:  6268567     Owner:  NLM     Status:  MEDLINE    
Seven Australian infectious bronchitis viruses incorporated radioactive amino acid label during growth in two types of cell culture. From purified virions, four major polypeptides of molecular weights 91,000 (GP91), 51,000 (VP51), 25,000 (GP25) and 23,000 (VP23) were separated by PAGE. Two minor polypeptides of molecular weights 210,000 (GP210) and 185,000 (GP185) were also identified. 3H-glucosamine was incorporated during growth of virus into GP210, GP185, GP91 and GP25. Bromelain digestion of virions removed GP210, GP185 and GP91, leaving VP51, GP25 and VP23 in a large subviral particle. All seven viruses, which were of six serological subtypes, formed apparently identical polypeptide profiles.
C N Wadey; E G Westaway
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Intervirology     Volume:  15     ISSN:  0300-5526     ISO Abbreviation:  Intervirology     Publication Date:  1981  
Date Detail:
Created Date:  1981-11-22     Completed Date:  1981-11-22     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0364265     Medline TA:  Intervirology     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  19-27     Citation Subset:  IM    
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MeSH Terms
Cells, Cultured
Chick Embryo
Coronaviridae / analysis*
Glycoproteins / analysis*
Infectious bronchitis virus / analysis*,  classification,  growth & development
Viral Proteins / analysis*
Virion / analysis
Reg. No./Substance:
0/Glycoproteins; 0/Viral Proteins

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