| Structural properties of lipopolysaccharides from Rickettsia typhi and Rickettsia prowazekii and their chemical similarity to the lipopolysaccharide from Proteus vulgaris OX19 used in the Weil-Felix test. | |
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MedLine Citation:
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PMID: 9488376 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The lipopolysaccharides (LPSs) isolated from typhus group (TG) rickettsiae Rickettsia typhi and Rickettsia prowazekii were characterized by chemical analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by silver staining. LPSs from two species of TG rickettsiae contained glucose, 3-deoxy-D-manno-octulosonic acid, glucosamine, quinovosamine, phosphate, and fatty acids (beta-hydroxylmyristic acid and heneicosanoic acid) but not heptose. The O-polysaccharides of these LPSs were composed of glucose, glucosamine, quinovosamine, and phosphorylated hexosamine. Resolution of these LPSs by their apparent molecular masses by SDS-PAGE showed that they have a common ladder-like pattern. Based on the results of chemical composition and SDS-PAGE pattern, we suggest that these LPSs act as group-specific antigens. Furthermore, glucosamine, quinovosamine, and phosphorylated hexosamine were also found in the O-polysaccharide of the LPS from Proteus vulgaris OX19 used in the Weil-Felix test, suggesting that they may represent the antigens common to LPSs from TG rickettsiae and P. vulgaris OX19. |
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Authors:
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K I Amano; J C Williams; G A Dasch |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Infection and immunity Volume: 66 ISSN: 0019-9567 ISO Abbreviation: Infect. Immun. Publication Date: 1998 Mar |
Date Detail:
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Created Date: 1998-03-12 Completed Date: 1998-03-12 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 923-6 Citation Subset: IM |
Affiliation:
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Airborne Diseases Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21701-5011, USA. amanocrl@med.akita-u.ac.jp |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, Bacterial
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immunology* Electrophoresis, Polyacrylamide Gel Epitopes Humans Lipopolysaccharides / chemistry*, immunology, isolation & purification Proteus vulgaris / chemistry*, immunology Rickettsia Infections / diagnosis* Rickettsia prowazekii / chemistry*, immunology Rickettsia typhi / chemistry*, immunology |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Bacterial; 0/Epitopes; 0/Lipopolysaccharides |
| Comments/Corrections | |
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