Document Detail

Structural and permeability characterization of biosynthetic PVA hydrogels designed for cell-based therapy.
MedLine Citation:
PMID:  25145396     Owner:  NLM     Status:  Publisher    
Incorporation of extracellular matrix (ECM) components to synthetic hydrogels has been shown to be the key for successful cell encapsulation devices, by providing a biofunctional microenvironment for the encapsulated cells. However, the influence of adding ECM components into synthetic hydrogels on the permeability as well as the physical and mechanical properties of the hydrogel has had little attention. Therefore, the aim of this study was to investigate the effect of incorporated ECM analogues on the permeability performance of permselective synthetic poly(vinyl alcohol) (PVA) hydrogels in addition to examining the physico-mechanical characteristics. PVA was functionalized with a systematically increased number of methacrylate functional groups per chain (FG/c) to tailor the permselectivity of UV photopolymerized hydrogel network. Heparin and gelatin were successfully incorporated into PVA network at low percentage (1%), and co-hydrogels were characterized for network properties and permeability to bovine serum albumin (BSA) and immunoglobulin G (IgG) proteins. Incorporation of these ECM analogues did not interfere with the base PVA network characteristics, as the controlled hydrogel mesh sizes, swelling and compressive modulii remained unchanged. While the permeation profiles of both BSA and IgG were not affected by the addition of heparin and gelatin as compared with pure PVA, increasing the FG/c from 7 to 20 significantly limited the diffusion of the larger IgG. Consequently, biosynthetic hydrogels composed of PVA with high FG/c and low percent ECM analogues show promise in their ability to be permselective for various biomedical applications.
Eman H Nafea; Laura A Poole-Warren; Penny J Martens
Related Documents :
2386886 - Established mouse liver cell lines as a model system for studying epithelio-mesenchymal...
17418236 - Identification and characterization of cmp-neuac:galnac-iga1 alpha2,6-sialyltransferase...
21152526 - Combination of fluid and solid mechanical stresses contribute to cell death and detachm...
20176206 - Modulation of growth in human esophageal adenocarcinoma cells by group iia secretory ph...
1333786 - Distinction of two different classes of small-cell lung cancer cell lines by enzymatica...
23037946 - Contribution of cells derived from the area pellucida to extraembryonic mesodermal cell...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-8-22
Journal Detail:
Title:  Journal of biomaterials science. Polymer edition     Volume:  -     ISSN:  1568-5624     ISO Abbreviation:  J Biomater Sci Polym Ed     Publication Date:  2014 Aug 
Date Detail:
Created Date:  2014-8-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9007393     Medline TA:  J Biomater Sci Polym Ed     Country:  -    
Other Details:
Languages:  ENG     Pagination:  1-20     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Distinct subclades of Aux/IAA genes are direct targets of ARF5/MP transcriptional regulation.
Next Document:  HemR is a OmpR/PhoB-like response regulator from Leptospira, which simultaneously effects transcript...