| Structural organization of a Bacillus subtilis operon encoding menaquinone biosynthetic enzymes. | |
MedLine Citation:
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PMID: 8566759 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Menaquinone (MK) is a non-protein component of the Bacillus subtilis (Bs) electron transport chain synthesized from chorismate through a series of MK-specific reactions. The genes encoding biosynthesis of the naphthoquinone ring of MK are clustered at 273 degrees on the Bs chromosome. A 3.9-kb region capable of rescuing men mutants blocked in the early stages of MK biosynthesis was sequenced and found to contain three major open reading frames (ORFs). The first ORF (menF) has a predicted size of 51.8 kDa and 34% amino-acid identity with the isochorismate synthases of Escherichia coli (EntC) and Aeromonas hydrophila (AmoA), ORF2 (menD) a predicted size of 60.2 kDa and 21% identity with MenD of E. coli. ORF3 has a predicted size of 21.4 kDa and 29% identity to triacylglycerol lipase of Psychrobacter immobilis. No sequence corresponding to menC was identified. Plasmid integrational studies of the men gene cluster had suggested the presence of promoters secondary to the previously identified p1 men promoter. Sequence analysis revealed a putative promoter region upstream from ORF3. |
Authors:
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B Rowland; K Hill; P Miller; J Driscoll; H Taber |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Gene Volume: 167 ISSN: 0378-1119 ISO Abbreviation: Gene Publication Date: 1995 Dec |
Date Detail:
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Created Date: 1996-03-01 Completed Date: 1996-03-01 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7706761 Medline TA: Gene Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 105-9 Citation Subset: IM |
Affiliation:
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Department of Microbiology, Immunology and Molecular Genetics, Albany Medical College, NY 12208, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/M74538 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus subtilis
/
genetics*,
metabolism Base Sequence Carboxy-Lyases / genetics Consensus Sequence Gene Expression Regulation, Bacterial Intramolecular Transferases* Isomerases / genetics Lipase / genetics Molecular Sequence Data Open Reading Frames Operon Oxo-Acid-Lyases / genetics RNA, Messenger / genetics Restriction Mapping Vitamin K / biosynthesis* |
| Grant Support | |
ID/Acronym/Agency:
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GM44547/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 12001-79-5/Vitamin K; EC 3.1.1.3/Lipase; EC 4.1.1.-/Carboxy-Lyases; EC 4.1.1.71/2-oxoglutarate decarboxylase; EC 4.1.3.-/2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase; EC 4.1.3.-/Oxo-Acid-Lyases; EC 5.-/Isomerases; EC 5.4.-/Intramolecular Transferases; EC 5.4.4.2/isochorismate synthase |
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