Document Detail

Structural organization of a Bacillus subtilis operon encoding menaquinone biosynthetic enzymes.
MedLine Citation:
PMID:  8566759     Owner:  NLM     Status:  MEDLINE    
Menaquinone (MK) is a non-protein component of the Bacillus subtilis (Bs) electron transport chain synthesized from chorismate through a series of MK-specific reactions. The genes encoding biosynthesis of the naphthoquinone ring of MK are clustered at 273 degrees on the Bs chromosome. A 3.9-kb region capable of rescuing men mutants blocked in the early stages of MK biosynthesis was sequenced and found to contain three major open reading frames (ORFs). The first ORF (menF) has a predicted size of 51.8 kDa and 34% amino-acid identity with the isochorismate synthases of Escherichia coli (EntC) and Aeromonas hydrophila (AmoA), ORF2 (menD) a predicted size of 60.2 kDa and 21% identity with MenD of E. coli. ORF3 has a predicted size of 21.4 kDa and 29% identity to triacylglycerol lipase of Psychrobacter immobilis. No sequence corresponding to menC was identified. Plasmid integrational studies of the men gene cluster had suggested the presence of promoters secondary to the previously identified p1 men promoter. Sequence analysis revealed a putative promoter region upstream from ORF3.
B Rowland; K Hill; P Miller; J Driscoll; H Taber
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Gene     Volume:  167     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  1995 Dec 
Date Detail:
Created Date:  1996-03-01     Completed Date:  1996-03-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  105-9     Citation Subset:  IM    
Department of Microbiology, Immunology and Molecular Genetics, Albany Medical College, NY 12208, USA.
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MeSH Terms
Bacillus subtilis / genetics*,  metabolism
Base Sequence
Carboxy-Lyases / genetics
Consensus Sequence
Gene Expression Regulation, Bacterial
Intramolecular Transferases*
Isomerases / genetics
Lipase / genetics
Molecular Sequence Data
Open Reading Frames
Oxo-Acid-Lyases / genetics
RNA, Messenger / genetics
Restriction Mapping
Vitamin K / biosynthesis*
Grant Support
Reg. No./Substance:
0/RNA, Messenger; 12001-79-5/Vitamin K; EC; EC 4.1.1.-/Carboxy-Lyases; EC decarboxylase; EC 4.1.3.-/2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase; EC 4.1.3.-/Oxo-Acid-Lyases; EC 5.-/Isomerases; EC 5.4.-/Intramolecular Transferases; EC synthase

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